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IGF1R Protein (AA 763-931, partial) (GST tag)

IGF1R Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Catalog No. ABIN5709725
  • Target See all IGF1R Proteins
    IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
    Protein Type
    Recombinant
    Protein Characteristics
    partial, AA 763-931
    Origin
    • 26
    • 6
    • 4
    • 2
    • 1
    Human
    Source
    • 14
    • 9
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This IGF1R protein is labelled with GST tag.
    Application
    SDS-PAGE (SDS)
    Sequence
    YNITDPEELE TEYPFFESRV DNKERTVISN LRPFTLYRID IHSCNHEAEK LGCSASNFVF ARTMPAEGAD DIPGPVTWEP RPENSIFLKW PEPENPNGLI LMYEIKYGSQ VEDQRECVSR QEYRKYGGAK LNRLNPGNYT ARIQATSLSG NGSWTDPVFF YVQAKTGYE
    Purification
    SDS-PAGE
    Purity
    > 90 %
    Top Product
    Discover our top product IGF1R Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.1-2 mg/mL
    Buffer
    20 mM Tris-HCl based buffer, pH 8.0
    Storage
    -80 °C,4 °C,-20 °C
    Storage Comment
    Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Target
    IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
    Alternative Name
    IGF1R (IGF1R Products)
    Synonyms
    CMT2N Protein, CD221 Protein, IGFIR Protein, IGFR Protein, JTK13 Protein, IGFIRC Protein, IGFR1 Protein, IGF-1R Protein, IGF1R Protein, DKFZp469I0618 Protein, igf-1r Protein, xIGF-1R Protein, xIGFR Protein, xigf1r Protein, A330103N21Rik Protein, D930020L01 Protein, hyft Protein, igf-ir Protein, IGF-IR Protein, IGF-IRb Protein, wu:fb03h06 Protein, IGF-IRa Protein, sb:eu1090 Protein, wu:fi20b10 Protein, alanyl-tRNA synthetase Protein, insulin like growth factor 1 receptor Protein, insulin-like growth factor 1 receptor Protein, insulin-like growth factor 1 Protein, insulin-like growth factor I receptor Protein, insulin like growth factor 1 receptor S homeolog Protein, insulin-like growth factor 1b receptor Protein, insulin-like growth factor 1a receptor Protein, AARS Protein, IGF1R Protein, Igf1r Protein, igf1r Protein, Igf1 Protein, LOC780503 Protein, LOC100136427 Protein, igf1r.S Protein, igf1rb Protein, igf1ra Protein
    Background
    Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
    Molecular Weight
    35.42 kDa
    UniProt
    P08069
    Pathways
    RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy
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