MCM2 Protein (AA 2-904) (His tag)

Catalog No. ABIN5709919

Product Details

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Protein Type: Recombinant

Protein Characteristics: AA 2-904

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MCM2 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: AESSESFTMA SSPAQRRRGN DPLTSSPGRS SRRTDALTSS PGRDLPPFED ESEGLLGTEG PLEEEEDGEE LIGDGMERDY RAIPELDAYE AEGLALDDED VEELTASQRE AAERAMRQRD REAGRGLGRM RRGLLYDSDE EDEERPARKR RQVERATEDG EEDEEMIESI ENLEDLKGHS VREWVSMAGP RLEIHHRFKN FLRTHVDSHG HNVFKERISD MCKENRESLV VNYEDLAARE HVLAYFLPEA PAELLQIFDE AALEVVLAMY PKYDRITNHI HVRISHLPLV EELRSLRQLH LNQLIRTSGV VTSCTGVLPQ LSMVKYNCNK CNFVLGPFCQ SQNQEVKPGS CPECQSAGPF EVNMEETIYQ NYQRIRIQES PGKVAAGRLP RSKDAILLAD LVDSCKPGDE IELTGIYHNN YDGSLNTANG FPVFATVILA NHVAKKDNKV AVGELTDEDV KMITSLSKDQ QIGEKIFASI APSIYGHEDI KRGLALALFG GEPKNPGGKH KVRGDINVLL CGDPGTAKSQ FLKYIEKVSS RAIFTTGQGA SAVGLTAYVQ RHPVSREWTL EAGALVLADR GVCLIDEFDK MNDQDRTSIH EAMEQQSISI SKAGIVTSLQ ARCTVIAAAN PIGGRYDPSL TFSENVDLTE PIISRFDILC VVRDTVDPVQ DEMLARFVVG SHVRHHPSNK EEEGLANGSA AEPAMPNTYG VEPLPQEVLK KYIIYAKERV HPKLNQMDQD KVAKMYSDLR KESMATGSIP ITVRHIESMI RMAEAHARIH LRDYVIEDDV NMAIRVMLES FIDTQKFSVM RSMRKTFARY LSFRRDNNEL LLFILKQLVA EQVTYQRNRF GAQQDTIEVP EKDLVDKARQ INIHNLSAFY DSELFRMNKF SHDLKRKMIL QQF

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Alternative Name: MCM2 (MCM2 Products)

Synonyms: CG7538 Protein, DmMCM2 Protein, DmMcm2 Protein, DmeMCM2 Protein, Dmel\\CG7538 Protein, MCM2 Protein, MCM2-7 Protein, MCM2_DROME Protein, McM2 Protein, PCR3 Protein, dMCM2 Protein, l(3)rL074 Protein, XMcm2 Protein, bm28 Protein, ccnl1 Protein, cdc19 Protein, cdcl1 Protein, mitotin Protein, cb737 Protein, chunp6905 Protein, BM28 Protein, CCNL1 Protein, CDCL1 Protein, D3S3194 Protein, MITOTIN Protein, MCM7 Protein, AA959861 Protein, AW476101 Protein, Mcmd2 Protein, mKIAA0030 Protein, Minichromosome maintenance 2 Protein, minichromosome maintenance complex component 2 Protein, minichromosome maintenance complex component 2 L homeolog Protein, Mcm2 Protein, mcm2 Protein, MCM2 Protein, mcm2.L Protein

Background: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

Molecular Weight: 105.8 kDa

UniProt: P49736

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA