OGT Protein (AA 606-1022, partial) (His-SUMO Tag)

Catalog No. ABIN5710043

Product Details

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Protein Type: Recombinant

Protein Characteristics: AA 606-1022, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This OGT protein is labelled with His-SUMO Tag.

Application: SDS-PAGE (SDS)

Sequence: MAEANHFIDL SQIPCNGKAA DRIHQDGIHI LVNMNGYTKG ARNELFALRP APIQAMWLGY PGTSGALFMD YIITDQETSP AEVAEQYSEK LAYMPHTFFI GDHANMFPHL KKKAVIDFKS NGHIYDNRIV LNGIDLKAFL DSLPDVKIVK MKCPDGGDNA DSSNTALNMP VIPMNTIAEA VIEMINRGQI QITINGFSIS NGLATTQINN KAATGEEVPR TIIVTTRSQY GLPEDAIVYC NFNQLYKIDP STLQMWANIL KRVPNSVLWL LRFPAVGEPN IQQYAQNMGL PQNRIIFSPV APKEEHVRRG QLADVCLDTP LCNGHTTGMD VLWAGTPMVT MPGETLASRV AASQLTCLGC LELIAKNRQE YEDIAVKLGT DLEYLKKVRG KVWKQRISSP LFNTKQYTME LERLYLQ

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Alternative Name: OGT1 (OGT Products)

Synonyms: BcDNA:GH04245 Protein, CG10392 Protein, Dmel\\CG10392 Protein, OGT Protein, Ogt Protein, P1201 Protein, SXC Protein, Sxc Protein, l(2)02637 Protein, l(2)NC130 Protein, sxc/Ogt Protein, fm81g08 Protein, ogt Protein, wu:fc12b01 Protein, wu:fm81g08 Protein, HRNT1 Protein, O-GLCNAC Protein, 1110038P24Rik Protein, 4831420N21Rik Protein, AI115525 Protein, Ogtl Protein, O-linked N-acetylglucosamine (GlcNAc) transferase L homeolog Protein, super sex combs Protein, O-linked N-acetylglucosamine (GlcNAc) transferase, tandem duplicate 1 Protein, O-linked N-acetylglucosamine (GlcNAc) transferase Protein, O-linked GlcNAc transferase Protein, o-linked GlcNAc transferase Protein, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) Protein, ogt.L Protein, sxc Protein, ogt.1 Protein, OGT Protein, ogt Protein, GL50803_12081 Protein, GL50803_41701 Protein, THAPSDRAFT_264441 Protein, spyA Protein, Ogt Protein

Background: Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity . Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3) . As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues . O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex . Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2

Molecular Weight: 62.5 kDa

UniProt: O15294

Pathways: Regulation of Carbohydrate Metabolic Process