SKP1 Protein (AA 2-163) (GST tag)

Catalog No. ABIN5710368

Product Details

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Protein Type: Recombinant

Protein Characteristics: AA 2-163

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SKP1 protein is labelled with GST tag.

Application: SDS-PAGE (SDS)

Sequence: PSIKLQSSDG EIFEVDVEIA KQSVTIKTML EDLGMDDEGD DDPVPLPNVN AAILKKVIQW CTHHKDDPPP PEDDENKEKR TDDIPVWDQE FLKVDQGTLF ELILAANYLD IKGLLDVTCK TVANMIKGKT PEEIRKTFNI KNDFTEEEEA QVRKENQWCE EK

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: SKP1 (SKP1 Products)

Synonyms: EMC19 Protein, OCP-II Protein, OCP2 Protein, SKP1A Protein, TCEB1L Protein, p19A Protein, 15kDa Protein, 2610043E24Rik Protein, 2610206H23Rik Protein, SKP1 Protein, Tceb1l Protein, p19Skp1 Protein, Skp1a Protein, skp1a Protein, zgc:73186 Protein, ocp2 Protein, emc19 Protein, ocp-ii Protein, tceb1l Protein, ARABIDOPSIS SKP1 HOMOLOGUE 1 Protein, ASK1 Protein, ATSKP1 Protein, S phase kinase-associated protein 1 Protein, T4O12.17 Protein, T4O12_17 Protein, UFO INTERACTING PROTEIN 1 Protein, UIP1 Protein, ocp-2 Protein, S-phase kinase associated protein 1 Protein, S-phase kinase-associated protein 1 Protein, S-phase kinase-associated protein 1 pseudogene Protein, S-phase kinase-associated protein 1A Protein, S-phase kinase-associated protein 1 S homeolog Protein, S phase kinase-associated protein 1 Protein, SKP1 Protein, skp1 Protein, LOC100340043 Protein, Skp1a Protein, Skp1 Protein, skp1.S Protein

Background: Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not se to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22', the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) direct ubiquitination of TTI1 and TELO2. SCF(FBXO10) direct ubiquitination of BCL2.

Molecular Weight: 34.52 kDa

UniProt: P63208

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases