RuvA Protein (AA 1-203, full length) (His-SUMO Tag)
Origin: E. coli
Host: Escherichia coli (E. coli)
Recombinant
> 90 %
SDS
1 image
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- Target See all RuvA products
- RuvA
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-203, full length
- Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RuvA protein is labelled with His-SUMO Tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MIGRLRGIII EKQPPLVLIE VGGVGYEVHM PMTCFYELPE AGQEAIVFTH FVVREDAQLL YGFNNKQERT LFKELIKTNG VGPKLALAIL SGMSAQQFVN AVEREEVGAL VKLPGIGKKT AERLIVEMKD RFKGLHGDLF TPAADLVLTS PASPATDDAE QEAVAALVAL GYKPQEASRM VSKIARPDAS SETLIREALR AAL
- Purification
- SDS-PAGE
- Purity
- > 90 %
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RuvA (Active) protein
Origin: E. coli Host: Escherichia coli (E. coli) Native Func Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- RuvA
- Abstract
- RuvA Products
- Background
- The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.
- Molecular Weight
- 38.07 kDa
- UniProt
- P0A809
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