SMUG1 Protein (AA 1-177, full length) (His-SUMO Tag)
-
- Target See all SMUG1 Proteins
- SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))
- Protein Type
- Recombinant
- Protein Characteristics
- full length, AA 1-177
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SMUG1 protein is labelled with His-SUMO Tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MPQAFLLGSI HEPAGALMEP QPCPGSLAES FLEEELRLNA ELSQLQFSEP VGIIYNPVEY AWEPHRNYVT RYCQGPKEVL FLGMNPGPFG MAQTGVPFGE VSMVRDWLGI VGPVLTPPQE HPKRPVLGLE CPQSEGPRQS MGHEIKSELL MGGCSWIRGK IQCDRVQVRR PGFSSQL
- Purification
- SDS-PAGE
- Purity
- > 90 %
- Top Product
- Discover our top product SMUG1 Protein
-
Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) (AA 1-177) protein (GST tag)
SMUG1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) (AA 2-78) protein (GST tag)SMUG1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) (AA 1-270) protein (Strep Tag)Crystallography grade SMUG1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) (AA 1-279) protein (Strep Tag)Crystallography grade SMUG1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) protein (Myc-DYKDDDDK Tag)SMUG1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
-
- Target
- SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))
- Alternative Name
- SMUG1 (SMUG1 Products)
- Synonyms
- SMUG1 Protein, xsmug1 Protein, 1200013B09Rik Protein, A930006H09Rik Protein, C85220 Protein, FDG Protein, HMUDG Protein, UNG3 Protein, single-strand-selective monofunctional uracil-DNA glycosylase 1 Protein, single-strand selective monofunctional uracil DNA glycosylase Protein, single-strand-selective monofunctional uracil-DNA glycosylase 1 L homeolog Protein, SMUG1 Protein, smug1 Protein, smuG1 Protein, smuG Protein, LOC5577584 Protein, CpipJ_CPIJ002767 Protein, Smug1 Protein, smug1.L Protein
- Background
- Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.
- Molecular Weight
- 35.61 kDa
- UniProt
- Q53HV7
- Pathways
- DNA Damage Repair
-
