ALKBH3 Protein (AA 1-170) (His-SUMO Tag)
-
- Target See all ALKBH3 Proteins
- ALKBH3 (AlkB, Alkylation Repair Homolog 3 (ALKBH3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-170
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ALKBH3 protein is labelled with His-SUMO Tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MEEKRRRARV QGAWAAPVKS QAIAQPATTA KSHLHQKPGQ TWKNKEHHLS DREFVFKEPQ QVVRRAPEPR VIEEGVYEIS LSPTGVSRVC LYPGFVDVKE ADWILEQLCQ DVPWKQRTGI REDSILQLTF KKSAPVSGTA TAPQSCWYER PSPPHIPGPA ILTRTRLWAP
- Purification
- SDS-PAGE
- Purity
- > 90 %
- Top Product
- Discover our top product ALKBH3 Protein
-
AlkB, Alkylation Repair Homolog 3 (ALKBH3) (AA 1-286) protein (His tag)
ALKBH3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
AlkB, Alkylation Repair Homolog 3 (ALKBH3) (AA 1-139) protein (GST tag)ALKBH3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
AlkB, Alkylation Repair Homolog 3 (ALKBH3) (AA 1-286) protein (Strep Tag)Crystallography grade ALKBH3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
AlkB, Alkylation Repair Homolog 3 (ALKBH3) (AA 1-286) protein (Strep Tag)Crystallography grade ALKBH3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
AlkB, Alkylation Repair Homolog 3 (ALKBH3) protein (Myc-DYKDDDDK Tag)ALKBH3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
-
- Target
- ALKBH3 (AlkB, Alkylation Repair Homolog 3 (ALKBH3))
- Alternative Name
- ALKB3 (ALKBH3 Products)
- Synonyms
- ABH3 Protein, DEPC-1 Protein, DEPC1 Protein, PCA1 Protein, 1700108H04Rik Protein, 1810020C19Rik Protein, Abh3 Protein, mABH3 Protein, alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase L homeolog Protein, alkB homolog 3, alpha-ketoglutaratedependent dioxygenase Protein, alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase Protein, alkbh3.L Protein, ALKBH3 Protein, Alkbh3 Protein
- Background
- Dioxygenase that repairs alkylated DNA containing 1-methyladenine (1meA) and 3-methylcytosine (3meC) by oxidative dethylation. Has a strong preference for single-stranded DNA. Able to process alkylated 3mC within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.
- Molecular Weight
- 35.3 kDa
- UniProt
- Q96Q83
- Pathways
- DNA Damage Repair
-
