Crossover junction endonuclease EME1 (EME1) (AA 1-583) protein (His-SUMO Tag)
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- Target See all Crossover junction endonuclease EME1 (EME1) Proteins
- Crossover junction endonuclease EME1 (EME1)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-583
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His-SUMO Tag
- Application
- SDS-PAGE (SDS)
- Sequence
- MALKKSSPSL DSGDSDSEEL PTFAFLKKEP SSTKRRQPER EEKIVVVDIS DCEASCPPAP ELFSPPVPDI AETVTQTQPV RLLSSESEDE EEFIPLAQRL TCKFLTHKQL SPEDSSSPVK SVLDHQNNEG ASCDWKKPFP KIPEVPLHDT PERSAADNKD LILDPCCQLP AYLSTCPGQS SSLAVTKTNS DILPPQKKTK PSQKVQGRGS HGCRQQRQAR QKESTLRRQE RKNAALVTRM KAQRPEECLK HIIVVLDPVL LQMEGGGQLL GALQTMECRC VIEAQAVPCS VTWRRRAGPS EDREDWVEEP TVLVLLRAEA FVSMIDNGKQ GSLDSTMKGK ETLQGFVTDI TAKTAGKALS LVIVDQEKCF SLELLFFDFL PCTSAQNPPR RGKQGANKQT KKQQQRQPEA SIGSMVSRVD AEEALVDLQL HTEAQAQIVQ SWKELADFTC AFTKAVAEAP FKKLRDETTF SFCLESDWAG GVKVDLAGRG LALVWRRQIQ QLNRVSLEMA SAVVNAYPSP QLLVQAYQQC FSDKERQNLL ADIQVRRGEG VTSTSRRIGP ELSRRIYLQM TTLQPHLSLD SAD
- Purification
- SDS-PAGE
- Purity
- > 90 %
- Top Product
- Discover our top product EME1 Protein
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Crossover junction endonuclease EME1 (EME1) (AA 1-583) protein (GST tag)
EME1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Crossover junction endonuclease EME1 (EME1) (AA 1-570) protein (Strep Tag)Crystallography grade EME1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Crossover junction endonuclease EME1 (EME1) (AA 1-570) protein (Strep Tag)Crystallography grade EME1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- Crossover junction endonuclease EME1 (EME1)
- Alternative Name
- EME1 (EME1 Products)
- Synonyms
- 6820428D13 Protein, essential meiotic structure-specific endonuclease 1 Protein, Eme1 Protein
- Background
- Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
- Molecular Weight
- 80.72 kDa
- UniProt
- Q96AY2
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