P4HTM Protein (AA 82-563) (His-SUMO Tag)
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- Target See all P4HTM Proteins
- P4HTM (Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 82-563
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This P4HTM protein is labelled with His-SUMO Tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- VHYSNGDESS DPGPQHRAQG PGPEPTLGPL TRLEGIKVGH ERKVQLVTDR DHFIRTLSLK PLLFEIPGFL TDEECRLIIH LAQMKGLQRS QILPTEEYEE AMSTMQVSQL DLFRLLDQNR DGHLQLREVL AQTRLGNGWW MTPESIQEMY AAIKADPDGD GVLSLQEFSN MDLRDFHKYM RSHKAESSEL VRNSHHTWLY QGEGAHHIMR AIRQRVLRLT RLSPEIVELS EPLQVVRYGE GGHYHAHVDS GPVYPETICS HTKLVANESV PFETSCRQVS PNWGLPSILR PGTPMTQAQP CTVGVPLGMG PGDHWVIPVS PWEHPQLGTC SVPPLPYSYM TVLFYLNNVT GGGETVFPVA DNRTYDEMSL IQDDVDLRDT RRHCDKGNLR VKPQQGTAVF WYNYLPDGQG WVGDVDDYSL HGGCLVTRGT KWIANNWINV DPSRARQALF QQEMARLARE GGTDSQPEWA LDRAYRDARV EL
- Purification
- SDS-PAGE
- Purity
- > 90 %
- Top Product
- Discover our top product P4HTM Protein
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Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (AA 1-563) protein (GST tag)
P4HTM Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (AA 82-502) protein (His tag)Crystallography grade P4HTM Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (AA 83-503) protein (His tag)Crystallography grade P4HTM Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (AA 1-503) protein (Strep Tag)Crystallography grade P4HTM Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (AA 1-502) protein (Strep Tag)Crystallography grade P4HTM Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- P4HTM (Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM))
- Alternative Name
- P4HTM (P4HTM Products)
- Synonyms
- Ph-4 Protein, RGD1311848 Protein, EGLN4 Protein, HIFPH4 Protein, P4H-TM Protein, PH-4 Protein, PH4 Protein, PHD4 Protein, 4933406E20Rik Protein, AI853847 Protein, BB128974 Protein, prolyl 4-hydroxylase, transmembrane Protein, prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) Protein, shisa family member 5 Protein, P4htm Protein, P4HTM Protein, SHISA5 Protein
- Background
- Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
- Molecular Weight
- 70.51 kDa
- UniProt
- Q9NXG6
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