TFPI2 Protein (His tag)
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- Target See all TFPI2 Proteins
- TFPI2 (Tissue Factor Pathway Inhibitor 2 (TFPI2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Tobacco (Nicotiana benthamiana)
- Purification tag / Conjugate
- This TFPI2 protein is labelled with His tag.
- Application
- Functional Studies (Func)
- Sequence
- HHHHHHGAAQ EPTGNNAEIC LLPLDYGPCK ALLLRYYYDR YTQSCRQFLY GGCEGNANNF YTWEACDDAC WRIEKVPKV
- Characteristics
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Molecular formula: C407H592N116O117S6.
Isoelectric Point: 6,25.
Extinction coefficient: E 0.1 % (1g/L) = 2.35 (A 280 nm).
This product contains no animal-derived components or impurities. It is produced by transient expression in non-transgenic plants. - Purification
- Recombinant AGV212 includes a 6 His-tag at the N-terminal end, is purified by sequential chromatography (FPLC).
- Purity
- > 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
- Endotoxin Level
- < 0.04 EU/µg protein (LAL method)
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Tissue Factor Pathway Inhibitor 2 (TFPI2) (AA 23-213) protein (His tag)
TFPI2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Tissue Factor Pathway Inhibitor 2 (TFPI2) (AA 1-235) protein (GST tag)TFPI2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tissue Factor Pathway Inhibitor 2 (TFPI2) (AA 23-235) protein (His tag)Crystallography grade TFPI2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Tissue Factor Pathway Inhibitor 2 (TFPI2) (AA 23-230) protein (His tag)Crystallography grade TFPI2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- The ability to prevent the hydrolysis of benzoyl-Larginine ethyl ester hydrochloride by trypsin.
- Comment
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Biological Activity: The activity of the inhibitor is expressed as the amount of trypsin inhibited per milligram of inhibitor. The ability to prevent the hydrolysis of benzoyl-L-arginine ethyl ester hydrochloride by trypsin is measured by spectrophotometer. One mg protein will inhibit 1-1.5 mg trypsin with activity of approximately 1000 BAEE units per mg protein.
Synonyms: First Kunitz domain of the human Tissue Factor Protein Inhibitor 2 (TFPI-2) protein, AGV 212 - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Lyophilized protein should be reconstituted adding 1 mL of sterile water to the vial, which gives a concentration of 1 mg of protease inhibitor per mL. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.
- Concentration
- 50 ng/µL
- Buffer
- PBS, pH 7.1.
- Storage
- 4 °C
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- Target
- TFPI2 (Tissue Factor Pathway Inhibitor 2 (TFPI2))
- Alternative Name
- TFPI-2 (TFPI2 Products)
- Synonyms
- MGC68843 Protein, TFPI2 Protein, MGC108301 Protein, si:ch211-262k23.2 Protein, tfpi2 Protein, PP5 Protein, REF1 Protein, TFPI-2 Protein, AV000670 Protein, PP5/TFPI-2 Protein, tissue factor pathway inhibitor 2 L homeolog Protein, tissue factor pathway inhibitor 2 Protein, tfpi2.L Protein, TFPI2 Protein, tfpi2 Protein, Tfpi2 Protein
- Background
- Recombinant human TFPI-2 doman 1 or AGV 212 is a protease inhibitor peptide generated from the first Kunitz domain of the human Tissue Factor Protein Inhibitor 2 (TFPI-2) protein, after site-directed mutagenesis to increase its activity. It is arranged in a single polypeptide chain that is linked by three disulfide bridges. AGV 212 is quite stable and inhibits trypsin with high efficiency and Ki lower than TFPI-2 one. TFPI-2 has been shown to inhibit Endothelial Cell Matrix (ECM) proteases essential for angiogenesis and metastasis.
- Molecular Weight
- 9.3 kDa
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