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Tubulin Protein (TUB) (Alexa Fluor 647)

TUB Origin: Cow Host: Cow Native > 99 % in vitro, SMMA, FSM, MI
Catalog No. ABIN5776683
  • Target See all Tubulin (TUB) Proteins
    Tubulin (TUB)
    Protein Type
    Native
    Origin
    Cow
    Source
    • 4
    Cow
    Purification tag / Conjugate
    This Tubulin protein is labelled with Alexa Fluor 647.
    Application
    In vitro Assay (in vitro), Single Molecule Motor Assays (SMMA), Fluorescent Speckle Microscopy (FSM), Microinjection (MI)
    Purpose
    Endogenous cytoskeletal protein
    Characteristics
    Polymerizes to form microtubules
    Purification
    Endogenous protein purified by differential centrifugation
    Purity
    > 99 %
    Top Product
    Discover our top product TUB Protein
  • Application Notes
    Tested for labeling stoichiometry (absorbance) and polymerization competency (fluorescence microscopy)
    Comment

    for use, but not limited to, in fluorescent experimental systems including: in vitro MT gliding assays and single molecule motor assays, speckle microscopy and live cell injection, in vitro nanoscale devices

    Protocol
    It is recommended to combine labeled tubulin with unlabeled tubulin at a ratio of 1:9 to a total tubulin concentration of 2 mg/mL
    Restrictions
    For Research Use only
  • Format
    Frozen
    Reconstitution
    Thaw rapidly at 37 °C and immediately place on ice
    Concentration
    20 mg/mL
    Buffer
    50 mM K-Glutamate and 0.5 mM MgCl2,  pH 7.0
    Preservative
    Without preservative
    Handling Advice
    protect from light
    Storage
    -80 °C
    Storage Comment

    Storage: -80°C

  • Target
    Tubulin (TUB)
    Alternative Name
    tubulin (TUB Products)
    Synonyms
    4t Protein, B4t Protein, BETA 98B Protein, CG4869 Protein, DTB1 Protein, Dmbeta4 Protein, Dmel\\CG4869 Protein, T Protein, Tub Protein, beta-Tub Protein, beta-Tub97EF Protein, beta-tub Protein, beta4 Protein, beta4t Protein, beta97F Protein, betaTub4 Protein, betaTub98BC Protein, betaTub98C Protein, beta-Tubulin at 97EF Protein, betaTub97EF Protein
    Molecular Weight
    ~110 kDa
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