Lactate Dehydrogenase A Protein (LDHA) (AA 1-332) (His tag)
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- Target See all Lactate Dehydrogenase A (LDHA) Proteins
- Lactate Dehydrogenase A (LDHA)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-332
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Origin
- Rat
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This Lactate Dehydrogenase A protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
- Top Product
- Discover our top product LDHA Protein
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Lactate Dehydrogenase A (LDHA) (AA 283-332) protein (His-IF2DI Tag)
LDHA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Lactate Dehydrogenase A (LDHA) (AA 5-323) protein (His tag)LDHA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Lactate Dehydrogenase A (LDHA) (AA 1-332) (Active) protein (His tag)LDHA Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Lactate Dehydrogenase A (LDHA) (AA 1-332) protein (GST tag)LDHA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Lactate Dehydrogenase A (LDHA) protein (His tag)LDHA Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Lactate Dehydrogenase A (LDHA) (AA 2-332) protein (His tag)Crystallography grade LDHA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lactate Dehydrogenase A (LDHA) (AA 2-332) protein (His tag)Crystallography grade LDHA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lactate Dehydrogenase A (LDHA) (AA 5-323), (partial) protein (His tag)LDHA Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Lactate Dehydrogenase A (LDHA) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)LDHA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, STD, PI Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 1 mM DTT, 20 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- Lactate Dehydrogenase A (LDHA)
- Alternative Name
- Ldha (LDHA Products)
- Synonyms
- GSD11 Protein, LDH1 Protein, LDHM Protein, LDH-M Protein, ldh-h Protein, ldha Protein, ldha-B Protein, ldhbb Protein, trg-5 Protein, Ldh1 Protein, Ldhm Protein, l7R2 Protein, gsd11 Protein, ldh-m Protein, ldh1 Protein, ldhm Protein, pig19 Protein, ldha1 Protein, ldhab Protein, LDH-A Protein, LDHC Protein, lactate dehydrogenase A Protein, lactate dehydrogenase B L homeolog Protein, lactate dehydrogenase A4 Protein, lactate dehydrogenase A L homeolog Protein, L-lactate dehydrogenase A chain Protein, LDHA Protein, ldhb.L Protein, Ldha Protein, ldha Protein, ldha.L Protein, LOC100713875 Protein
- Background
- Ldha, also known as lactate dehydrogenase A, catalyzes the conversion of pyruvate to lactate, utilizing NADH as a cofactor. It has been identified as a potential therapeutic target in the area of cancer metabolism. Reduction in Ldha activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. Recombinant rat Ldha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 37.5kDa (340aa) 28-40KDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_058721
- UniProt
- P04642
- Pathways
- Warburg Effect
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