SERPINC1 Protein (AA 1-546) (His tag)
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- Target See all SERPINC1 Proteins
- SERPINC1 (Serpin Family C Member 1 (SERPINC1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-546
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This SERPINC1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADPHGSPVDI CTAKPRDIPM NPMCIYRSPE KKATEDEGSE QKIPEATNRR VWELSKANSR FATTFYQHLA DSKNDNDNIF LSPLSISTAF AMTKLGACND TLQQLMEVFK FDTISEKTSD QIHFFFAKLN CRLYRKANKS SKLVSANRLF GDKSLTFNET YQDISELVYG AKLQPLDFKE NAEQSRAAIN KWVSNKTEGR ITDVIPSEAI NELTVLVLVN TIYFKGLWKS KFSPENTRKE LFYKADGESC SASMMYQEGK FRYRRVAEGT QVLELPFKGD DITMVLILPK PEKSLAKVEK ELTPEVLQEW LDELEEMMLV VHMPRFRIED GFSLKEQLQD MGLVDLFSPE KSKLPGIVAE GRDDLYVSDA FHKAFLEVNE EGSEAAASTA VVIAGRSLNP NRVTFKANRP FLVFIREVPL NTIIFMGRVA NPCVKHHHHH H
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 1nM.
- Top Product
- Discover our top product SERPINC1 Protein
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Serpin Family C Member 1 (SERPINC1) (AA 1-259) protein (GST tag)
SERPINC1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Serpin Family C Member 1 (SERPINC1) (AA 33-464) protein (His tag)Crystallography grade SERPINC1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Serpin Family C Member 1 (SERPINC1) (AA 33-465) protein (His tag)Crystallography grade SERPINC1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- SERPINC1 (Serpin Family C Member 1 (SERPINC1))
- Alternative Name
- SERPINC1 (SERPINC1 Products)
- Synonyms
- AT3 Protein, AT3D Protein, ATIII Protein, THPH7 Protein, SERPINC1 Protein, DKFZp470C1733 Protein, AI114908 Protein, At-3 Protein, At3 Protein, ANTITHROMBIN, AT-III Protein, AT-III Protein, serpin family C member 1 Protein, antithrombin-III Protein, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 Protein, SERPINC1 Protein, CpipJ_CPIJ000472 Protein, CpipJ_CPIJ013111 Protein, Serpinc1 Protein
- Background
- SERPINC1, also known as antithrombin-III, is a member of the Serpin superfamily of the serine protease inhibitors. It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa. It is one of the most important coagulation inhibitors and is the basic enzyme for the therapeutic action of heparin. In common with SerpinA5 and D1, the inhibitory activity of SerpinC1 dramatically increases the presence of heparin and other glycosaminoglycans. It mediates the promotion of prostaglandin release, inhibitors of leukocyte activation and down-regulation of many inflammatory cytokines. This protein has anti-inflammatory properties besides anticoagulant. Recombinant human SERPINC1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 50.1kDa (441aa) 50-70kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_000479
- UniProt
- P01008
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