PPIB Protein (AA 34-216) (His tag)

Catalog No. ABIN6388040

Product Details

Target: PPIB (Cyclophilin B (PPIB))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 34-216

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PPIB protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNDKKKG PKVTVKVYFD LQIGDESVGR VVFGLFGKTV PKTVDNFVAL ATGEKGFGYK NSKFHRVIKD FMIQGGDFTR GDGTGGKSIY GERFPDENFK LKHYGPGWVS MANAGKDTNG SQFFITTVKT SWLDGKHVVF GKVLEGMDVV RKVESTKTDS RDKPLKDVII VDSGKIEVEK PFAIAKE

Purity: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 1,000 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: PPIB (Cyclophilin B (PPIB))

Alternative Name: Ppib (PPIB Products)

Synonyms: cypb Protein, scylp Protein, cyp-s1 Protein, PPIB Protein, Ppib Protein, ACYPI004891 Protein, AA408962 Protein, AA553318 Protein, AI844835 Protein, Cphn-2 Protein, Cphn2 Protein, CyP-20b Protein, CypB Protein, Scylp Protein, CYP-S1 Protein, CYPB Protein, OI9 Protein, SCYLP Protein, cb87 Protein, sb:cb87 Protein, wu:fa97f08 Protein, zgc:73214 Protein, zgc:86796 Protein, peptidylprolyl isomerase B Protein, peptidylprolyl isomerase B (cyclophilin B) Protein, peptidylprolyl isomerase B L homeolog Protein, ppib Protein, PPIB Protein, Ppib Protein, CC1G_03223 Protein, ppib.L Protein

Background: Ppib, also known as peptidyl-prolyl cis-trans isomerase B, is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A -mediated immunosuppression. Recombinant mouse Ppib, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 22.7 kDa (207aa), confirmed by MALDI-TOF

NCBI Accession: NP_035279

UniProt: P24369