ENTPD3 Protein (AA 44-485) (His tag)
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- Target See all ENTPD3 Proteins
- ENTPD3 (Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 44-485
- Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This ENTPD3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADLQIHKQEV LPPGLKYGIV LDAGSSRTTV YVYQWPAEKE NNTGVVSQTF KCSVKGSGIS SYGNNPQDVP RAFEECMQKV KGQVPSHLHG STPIHLGATA GMRLLRLQNE TAANEVLESI QSYFKSQPFD FRGAQIISGQ EEGVYGWITA NYLMGNFLEK NLWHMWVHPH GVETTGALDL GGASTQISFV AGEKMDLNTS DIMQVSLYGY VYTLYTHSFQ CYGRNEAEKK FLAMLLQNSP TKNHLTNPCY PRDYSISFTM GHVFDSLCTV DQRPESYNPN DVITFEGTGD PSLCKEKVAS IFDFKACHDQ ETCSFDGVYQ PKIKGPFVAF AGFYYTASAL NLSGSFSLDT FNSSTWNFCS QNWSQLPLLL PKFDEVYARS YCFSANYIYH LFVNGYKFTE ETWPQIHFEK EVGNSSIAWS LGYMLSLTNQ IPAESPLIRL PIEPPHHHHH H
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 250,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze ATP per minute at pH 7.5 at 37C.
- Top Product
- Discover our top product ENTPD3 Protein
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Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3) (AA 1-452) protein (GST tag)
ENTPD3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3) (AA 107-216) protein (GST tag)ENTPD3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3) (AA 44-485) protein (His tag)Crystallography grade ENTPD3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3) (AA 1-529) protein (Strep Tag)Crystallography grade ENTPD3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3) protein (Myc-DYKDDDDK Tag)ENTPD3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- ENTPD3 (Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3))
- Alternative Name
- ENTPD3 (ENTPD3 Products)
- Synonyms
- ENTPD3 Protein, fi38a03 Protein, wu:fi38a03 Protein, zgc:162166 Protein, CD39L3 Protein, HB6 Protein, NTPDase-3 Protein, Cd39l3 Protein, NTPDase3 Protein, ectonucleoside triphosphate diphosphohydrolase 3 Protein, ectonucleoside triphosphate diphosphohydrolase 3 L homeolog Protein, ENTPD3 Protein, entpd3.L Protein, entpd3 Protein, Entpd3 Protein
- Background
- ENTPD3, also known as ectonucleoside triphosphate diphosphohydrolase 3, is an integral membrane glycoprotein with an extracellular active site. It contains 4 apyrase-conserved regions which is characteristic of NTPases. Recombinant human ENTPD3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 50.7kDa (451aa) 50-70KDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_001239
- UniProt
- O75355
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