Acad8 Protein (AA 23-415) (His tag)
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- Target See all Acad8 Proteins
- Acad8 (Acyl-CoA Dehydrogenase Family, Member 8 (Acad8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-415
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Acad8 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSLVQTGHR SLTSCIDPSM GLNEEQKEFQ KVAFDFAARE MAPNMAEWDQ KELFPVDVMR KAAQLGFGGV YIQTDVGGSG LSRLDTSVIF EALATGCTST TAYISIHNMC AWMIDSFGNE EQRHKFCPPL CTMEKFASYC LTEPGSGSDA ASLLTSAKKQ GDHYILNGSK AFISGAGESD IYVVMCRTGG PGPKGISCIV VEKGTPGLSF GKKEKKVGWN SQPTRAVIFE DCAVPVANRI GSEGQGFLIA VRGLNGGRIN IASCSLGAAH ASVILTRDHL NVRKQFGEPL ASNQYLQFTL ADMATRLVAA RLMVRNAAVA LQEERKDAVA LCSMAKLFAT DECFAICNQA LQMHGGYGYL KDYAVQQYVR DSRVHQILEG SNEVMRILIS RSLLQE
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product Acad8 Protein
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Acyl-CoA Dehydrogenase Family, Member 8 (Acad8) (AA 23-415) protein (His tag)
Acad8 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Acyl-CoA Dehydrogenase Family, Member 8 (Acad8) (AA 1-415) protein (GST tag)Acad8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Acyl-CoA Dehydrogenase Family, Member 8 (Acad8) (AA 306-415) protein (GST tag)Acad8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Acyl-CoA Dehydrogenase Family, Member 8 (Acad8) (AA 23-415) protein (His tag)Crystallography grade Acad8 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Acyl-CoA Dehydrogenase Family, Member 8 (Acad8) (AA 21-413) protein (His tag)Crystallography grade Acad8 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Acyl-CoA Dehydrogenase Family, Member 8 (Acad8) protein (Myc-DYKDDDDK Tag)Acad8 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 30 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- Acad8 (Acyl-CoA Dehydrogenase Family, Member 8 (Acad8))
- Alternative Name
- ACAD8 (Acad8 Products)
- Synonyms
- zgc:55874 Protein, DDBDRAFT_0219367 Protein, DDBDRAFT_0237710 Protein, DDB_0219367 Protein, DDB_0237710 Protein, ACAD-8 Protein, ARC42 Protein, RGD1564209 Protein, 2310016C19Rik Protein, AI786953 Protein, acyl-CoA dehydrogenase family member 8 L homeolog Protein, acyl-CoA dehydrogenase family, member 8 Protein, acyl-CoA dehydrogenase family member 8 Protein, acyl-CoA dehydrogenase Protein, acyl-Coenzyme A dehydrogenase family, member 8 Protein, acad8.L Protein, acad8 Protein, ACAD8 Protein, LOC100231097 Protein, Acad8 Protein
- Background
- ACAD8 is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. The protein is a mitochondrial enzyme that functions in catabolism of the branched-chain amino acid valine. Defects in this gene are the cause of isobutyryl-CoA dehydrogenase deficiency. Recombinant human ACAD8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 45.1kDa (416aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_055199
- UniProt
- Q9UKU7
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