GDA Protein (AA 1-454) (His tag)
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- Target See all GDA Proteins
- GDA (Guanine Deaminase (GDA))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-454
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GDA protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMCAARTP PLALVFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE SSQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHAPQ YAFAGSNVDL PLLEWLNKYT FPTEQRFRST DVAEEVYTRV VRRTLKNGTT TACYFGTIHT DSSLILAEIT DKFGQRAFVG KVCMDLNDTV PEYKETTEES VKETERFVSE MLQKNYPRVK PIVTPRFTLS CTETLMSELG NIAKTHDLYI QSHISENREE IEAVKSLYPS YKNYTDVYDK NNLLTNKTVM AHGCYLSEEE LNIFSERGAS IAHCPNSNLS LSSGLLNVLE VLKHKVKIGL GTDVAGGYSY SMLDAIRRAV MVSNVLLINK VNEKNLTLKE VFRLATLGGS QALGLDSEIG NFEVGKEFDA LLINPRASDS PIDLFYGDFV GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.
- Top Product
- Discover our top product GDA Protein
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Guanine Deaminase (GDA) (AA 1-454) protein (GST tag)
GDA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Guanine Deaminase (GDA) (AA 1-454) protein (His tag)GDA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Guanine Deaminase (GDA) (AA 1-454) protein (Strep Tag)Crystallography grade GDA Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Guanine Deaminase (GDA) (AA 1-454) protein (Strep Tag)Crystallography grade GDA Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Guanine Deaminase (GDA) (AA 1-454) (Active) proteinGDA Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS Active
Guanine Deaminase (GDA) protein (Myc-DYKDDDDK Tag)GDA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl( pH 8.0) containing 0.15M NaCl, 1 mM DTT, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- GDA (Guanine Deaminase (GDA))
- Alternative Name
- Gda (GDA Products)
- Synonyms
- CYPIN Protein, GUANASE Protein, NEDASIN Protein, AU015411 Protein, AW047581 Protein, Cypin Protein, MGC81835 Protein, zgc:112282 Protein, AGR_C_4212 Protein, DDBDRAFT_0169373 Protein, DDBDRAFT_0230211 Protein, DDB_0169373 Protein, DDB_0230211 Protein, guanine deaminase Protein, guanine deaminase L homeolog Protein, short-chain dehydrogenase/reductase sdr Protein, GDA Protein, Gda Protein, gda.L Protein, gda Protein, SPO2956 Protein, Gdia_3192 Protein, Mchl_2046 Protein, Mnod_4655 Protein, guaD Protein, Dd586_1771 Protein, Kvar_2512 Protein, Mrub_1462 Protein, BC1002_2263 Protein, Cseg_1079 Protein, Mesil_1795 Protein, Trad_2850 Protein, Mesop_0346 Protein, gDA Protein, ML5_2802 Protein
- Background
- Gda, also known as guanine deaminase, catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant mouse Gda, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
- Molecular Weight
- 53.4 kDa (477aa) Confirmed by MALDI-TOF
- NCBI Accession
- NP_034396
- UniProt
- Q9R111
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