IMPACT Protein (AA 1-320) (His tag)
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- Target See all IMPACT Proteins
- IMPACT (Impact Homolog (IMPACT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-320
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IMPACT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMAEGDAG SDQRQNEEIE AMAAIYGEEW CVIDDCAKIF CIRISDDIDD PKWTLCLQVM LPNEYPGTAP PIYQLNAPWL KGQERADLSN SLEEIYIQNI GESILYLWVE KIRDVLIQKS QMTEPGPDVK KKTEEEDVEC EDDLILACQP ESSVKALDFD ISETRTEVEV EELPPIDHGI PITDRRSTFQ AHLAPVVCPK QVKMVLSKLY ENKKIASATH NIYAYRIYCE DKQTFLQDCE DDGETAAGGR LLHLMEILNV KNVMVVVSRW YGGILLGPDR FKHINNCARN ILVEKNYTNS PEESSKALGK NKKVRKDKKR NEH
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product IMPACT Protein
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Impact Homolog (IMPACT) (AA 1-320) protein (His tag)
IMPACT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Impact Homolog (IMPACT) (AA 1-320) protein (GST tag)IMPACT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Impact Homolog (IMPACT) (AA 1-320) protein (Strep Tag)Crystallography grade IMPACT Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Impact Homolog (IMPACT) (AA 1-318) protein (Strep Tag)Crystallography grade IMPACT Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Impact Homolog (IMPACT) protein (Myc-DYKDDDDK Tag)IMPACT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- IMPACT (Impact Homolog (IMPACT))
- Alternative Name
- IMPACT (IMPACT Products)
- Synonyms
- zgc:103672 Protein, Ximpact Protein, impact-A Protein, RWDD5 Protein, impact-a Protein, E430016J11Rik Protein, impact RWD domain protein Protein, impact Protein, impact RWD domain protein L homeolog Protein, impact, RWD domain protein Protein, IMPACT Protein, impact Protein, EDI_298310 Protein, impact.L Protein, Impact Protein
- Background
- IMPACT belongs to the IMPACT family and contains 1 RWD domain. This protein is translational regulator that ensures constant high levels of translation under amino acid starvation and acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. Recombinant human IMPACT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 38.9kDa (343aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_060909
- UniProt
- Q9P2X3
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