MDH2 Protein (AA 25-338) (His tag)
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- Target See all MDH2 Proteins
- MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 25-338
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MDH2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET RANVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMVCIIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL ATLTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVQSKET ECTYFSTPLL LGKKGLEKNL GIGKITPFEE KMIAEAIPEL KASIKKGEDF VKNMK
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 800 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C
- Top Product
- Discover our top product MDH2 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In Phosphate buffer saline ( pH 7.4) containing 20 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))
- Alternative Name
- Mdh2 (MDH2 Products)
- Synonyms
- Mdh2b Protein, m-mdh Protein, mdh2 Protein, mor1 Protein, MDH-2 Protein, Mdh Protein, wu:fj48c08 Protein, wu:fj55d06 Protein, zgc:64133 Protein, M-MDH Protein, MDH Protein, MGC:3559 Protein, MOR1 Protein, MMDH Protein, Mdh-2 Protein, Mor-1 Protein, Mor1 Protein, malate dehydrogenase 2 S homeolog Protein, malate dehydrogenase 2 Protein, Malate dehydrogenase 2 Protein, malate dehydrogenase Protein, malate dehydrogenase 2, NAD (mitochondrial) Protein, mdh2.S Protein, MDH2 Protein, Mdh2 Protein, MDH4 Protein, mdh2 Protein
- Background
- Mdh2, also known as Malate dehydrogenase 2. Mdh2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, Mdh2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant mouse Mdh2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 35.4 kDa (335aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_032643
- UniProt
- P08249
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