ARFIP2 Protein (His tag)
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- Target See all ARFIP2 Proteins
- ARFIP2 (ADP-Ribosylation Factor Interacting Protein 2 (ARFIP2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ARFIP2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product ARFIP2 Protein
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ADP-Ribosylation Factor Interacting Protein 2 (ARFIP2) (AA 1-341) protein (His tag)
ARFIP2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
ADP-Ribosylation Factor Interacting Protein 2 (ARFIP2) (AA 1-341) protein (GST tag)ARFIP2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ADP-Ribosylation Factor Interacting Protein 2 (ARFIP2) (AA 1-110) protein (GST tag)ARFIP2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ADP-Ribosylation Factor Interacting Protein 2 (ARFIP2) (AA 1-341) protein (Strep Tag)Crystallography grade ARFIP2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
ADP-Ribosylation Factor Interacting Protein 2 (ARFIP2) protein (Myc-DYKDDDDK Tag)ARFIP2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Arfaptin 2, POR1
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 40% glycerol,1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- ARFIP2 (ADP-Ribosylation Factor Interacting Protein 2 (ARFIP2))
- Alternative Name
- ARFIP2 (ARFIP2 Products)
- Synonyms
- POR1 Protein, 2310002N04Rik Protein, POR Protein, Por1 Protein, ARFIP2 Protein, im:7136059 Protein, zgc:112066 Protein, ADP ribosylation factor interacting protein 2 Protein, ADP-ribosylation factor interacting protein 2 Protein, ADP-ribosylation factor interacting protein 2b Protein, ARFIP2 Protein, Arfip2 Protein, arfip2b Protein
- Background
- Arfaptin 2, also known as ARFIP2, is a Rac1 binding protein necessary for Rac-mediated actin polymerization and the subsequent formation of membrane ruffles and lamellipodia. ARFIP2 has also been shown to interact with the ADP ribosylation factor ARF6, a GTPase that associates with the plasma membrane and intracellular endosome vesicles, in a GTP dependent manner. Arfaptin 2 also regulates the aggregation of mutant Huntingtin protein by possibly impairing proteasome function. Expression of ARFIP2 was shown to be increased at sites of neurodegeneration. Recombinant human ARFIP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 40.2 kDa (364aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_036534
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