ACOT8 Protein (AA 1-319) (His tag)
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- Target See all ACOT8 Proteins
- ACOT8 (Acyl-CoA Thioesterase 8 (ACOT8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-319
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ACOT8 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMSSPQAP EDGQGCGDRG DPPGDLRSVL VTTVLNLEPL DEDLFRGRHY WVPAKRLFGG QIVGQALVAA AKSVSEDVHV HSLHCYFVRA GDPKLPVLYQ VERTRTGSSF SVRSVKAVQH GKPIFICQAS FQQAQPSPMQ HQFSMPTVPP PEELLDCETL IDQYLRDPNL QKRYPLALNR IAAQEVPIEI KPVNPSPLSQ LQRMEPKQMF WVRARGYIGE GDMKMHCCVA AYISDYAFLG TALLPHQWQH KVHFMVSLDH SMWFHAPFRA DHWMLYECES PWAGGSRGLV HGRLWRQDGV LAVTCAQEGV IRVKPQVSES KL
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product ACOT8 Protein
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Acyl-CoA Thioesterase 8 (ACOT8) (AA 1-319) protein (His tag)
ACOT8 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Acyl-CoA Thioesterase 8 (ACOT8) (AA 1-319) protein (GST tag)ACOT8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Acyl-CoA Thioesterase 8 (ACOT8) (AA 108-215) protein (GST tag)ACOT8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Acyl-CoA Thioesterase 8 (ACOT8) (AA 1-319) protein (Strep Tag)Crystallography grade ACOT8 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Acyl-CoA Thioesterase 8 (ACOT8) (AA 1-320) protein (Strep Tag)Crystallography grade ACOT8 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 40 % glycerol, 2 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- ACOT8 (Acyl-CoA Thioesterase 8 (ACOT8))
- Alternative Name
- ACOT8 (ACOT8 Products)
- Synonyms
- PTE1 Protein, zgc:101691 Protein, HNAACTE Protein, PTE-1 Protein, PTE-2 Protein, PTE2 Protein, hACTE-III Protein, hTE Protein, Pte1 Protein, acyl-CoA thioesterase 8 Protein, ACOT8 Protein, acot8 Protein, Acot8 Protein
- Background
- Acyl-coenzyme A thioesterase 8, also known as ACOT8, are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. This protein may mediate Nef-induced down-regulation of CD4. It competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). ACOT8 shows a preference for medium-length fatty acyl-CoAs. Recombinant human ACOT8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 38.3 kDa (342aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_005460
- UniProt
- O14734
- Pathways
- Monocarboxylic Acid Catabolic Process
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