RNASEH2A Protein (AA 1-299) (His tag)
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- Target See all RNASEH2A Proteins
- RNASEH2A (Ribonuclease H2, Subunit A (RNASEH2A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-299
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RNASEH2A protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMDLSELE RDNTGRCRLS SPVPAVCRKE PCVLGVDEAG RGPVLGPMVY AICYCPLPRL ADLEALKVAD SKTLLESERE RLFAKMEDTD FVGWALDVLS PNLISTSMLG RVKYNLNSLS HDTATGLIQY ALDQGVNVTQ VFVDTVGMPE TYQARLQQSF PGIEVTVKAK ADALYPVVSA ASICAKVARD QAVKKWQFVE KLQDLDTDYG SGYPNDPKTK AWLKEHVEPV FGFPQFVRFS WRTAQTILEK EAEDVIWEDS ASENQEGLRK ITSYFLNEGS QARPRSSHRY FLERGLESAT SL
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product RNASEH2A Protein
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Ribonuclease H2, Subunit A (RNASEH2A) (AA 1-299) protein (GST tag)
RNASEH2A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Ribonuclease H2, Subunit A (RNASEH2A) (AA 1-299) protein (Strep Tag)Crystallography grade RNASEH2A Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Ribonuclease H2, Subunit A (RNASEH2A) (AA 1-301) protein (Strep Tag)Crystallography grade RNASEH2A Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Ribonuclease H2, Subunit A (RNASEH2A) protein (Myc-DYKDDDDK Tag)RNASEH2A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M urea, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- RNASEH2A (Ribonuclease H2, Subunit A (RNASEH2A))
- Alternative Name
- RNASEH2A (RNASEH2A Products)
- Synonyms
- 2400006P09Rik Protein, RNASEHI Protein, RNHIA Protein, RNHL Protein, zgc:56307 Protein, AGS4 Protein, JUNB Protein, ribonuclease H2 subunit A Protein, ribonuclease H2, large subunit Protein, ribonuclease H2, subunit A Protein, ribonuclease H2 subunit A L homeolog Protein, rnaseh2a Protein, RNASEH2A Protein, Rnaseh2a Protein, rnaseh2a.L Protein
- Background
- Ribonuclease H2 subunit A, also known as RNASEH2A, belongs to the RNase HII family and eukaryotic subfamily. Localized to the nucleus, RNASEH2A mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. RNASEH2A catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4). Recombinant human RNASEH2A protein, fused to His-tag at N-terminus, was expressed in E.coli
- Molecular Weight
- 35.8kDa (322aa)
- NCBI Accession
- NP_006388
- UniProt
- O75792
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