POLE3 Protein (AA 1-147) (His tag)
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- Target See all POLE3 Proteins
- POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-147
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This POLE3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMAERPED LNLPNAVITR IIKEALPDGV NISKEARSAI SRAASVFVLY ATSCANNFAM KGKRKTLNAS DVLSAMEEME FQRFVTPLKE ALEAYRREQK GKKEASEQKK KDKDKKTDSE EQDKSRDEDN DEDEERLEEE EQNEEEEVDN
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product POLE3 Protein
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Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) (AA 1-147) protein (His tag)
POLE3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) (AA 1-147) protein (GST tag)POLE3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) (AA 2-147) protein (His tag)Crystallography grade POLE3 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) (AA 2-145) protein (His tag)Crystallography grade POLE3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) (AA 1-147) proteinPOLE3 Origin: Human Host: Escherichia coli (E. coli) Recombinant 95 % ELISA, WB, MS
Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) protein (Myc-DYKDDDDK Tag)POLE3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 20 % glycerol, 1 mM DTT.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))
- Alternative Name
- POLE3 (POLE3 Products)
- Synonyms
- NCU04548.1 Protein, GB14966 Protein, zgc:73351 Protein, 1810034K18Rik Protein, YBL1 Protein, CHARAC17 Protein, CHRAC17 Protein, p17 Protein, DNA polymerase epsilon 3, accessory subunit Protein, polymerase (DNA directed), epsilon 3, accessory subunit Protein, DNA polymerase epsilon Protein, DNA polymerase epsilon p17 subunit Protein, DNA polymerase epsilon subunit 3 Protein, polymerase (DNA directed), epsilon 3 (p17 subunit) Protein, POLE3 Protein, pole3 Protein, NCU04548 Protein, CpipJ_CPIJ008666 Protein, Bm1_04865 Protein, Bm1_07285 Protein, CTRG_00708 Protein, LOAG_09571 Protein, LOC726923 Protein, Pole3 Protein
- Background
- POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging. POLE3 has been shown to interact with SMARCA5. Recombinant human POLE3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 19kDa (170aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_059139
- UniProt
- Q9NRF9
- Pathways
- DNA Damage Repair
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