CNOT8 Protein (AA 1-292) (His tag)
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- Target See all CNOT8 Proteins
- CNOT8 (CCR4-NOT Transcription Complex, Subunit 8 (CNOT8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-292
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CNOT8 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMPAALVE NSQVICEVWA SNLEEEMRKI REIVLSYSYI AMDTEFPGVV VRPIGEFRSS IDYQYQLLRC NVDLLKIIQL GLTFTNEKGE YPSGINTWQF NFKFNLTEDM YSQDSIDLLA NSGLQFQKHE EEGIDTLHFA ELLMTSGVVL CDNVKWLSFH SGYDFGYMVK LLTDSRLPEE EHEFFHILNL FFPSIYDVKY LMKSCKNLKG GLQEVADQLD LQRIGRQHQA GSDSLLTGMA FFRMKELFFE DSIDDAKYCG RLYGLGTGVA QKQNEDVDSA QEKMSILAII NNMQQ
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product CNOT8 Protein
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CCR4-NOT Transcription Complex, Subunit 8 (CNOT8) (AA 1-292) protein (His tag)
CNOT8 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
CCR4-NOT Transcription Complex, Subunit 8 (CNOT8) (AA 1-292) protein (GST tag)CNOT8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
CCR4-NOT Transcription Complex, Subunit 8 (CNOT8) (AA 201-292) protein (GST tag)CNOT8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
CCR4-NOT Transcription Complex, Subunit 8 (CNOT8) (AA 1-292) protein (Strep Tag)Crystallography grade CNOT8 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
CCR4-NOT Transcription Complex, Subunit 8 (CNOT8) (AA 1-292) protein (Strep Tag)Crystallography grade CNOT8 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CNOT8 (CCR4-NOT Transcription Complex, Subunit 8 (CNOT8))
- Alternative Name
- CNOT8 (CNOT8 Products)
- Synonyms
- calif Protein, pop2 Protein, zgc:63844 Protein, wu:fe49a05 Protein, caf1 Protein, hcaf1 Protein, CAF1 Protein, CALIF Protein, Caf1b Protein, POP2 Protein, hCAF1 Protein, 1500015I04Rik Protein, 1810022F04Rik Protein, AA536816 Protein, AU015770 Protein, AU043059 Protein, CCR4-NOT transcription complex, subunit 8 Protein, CCR4-NOT transcription complex subunit 8 Protein, CCR4-NOT transcription complex subunit 8 S homeolog Protein, Cnot8 Protein, CNOT8 Protein, cnot8.S Protein, cnot8 Protein, PAAG_04884 Protein, VDBG_08730 Protein
- Background
- CNOT8 belongs to the CAF1 family. It is a ubiquitous transcription factor required for a diverse set of processes and functions as part of the CCR-NOT complex. The CCR4-NOT complex functions as general transcription regulation complex. This complex contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8 and binds BTG2. Recombinant human CNOT8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 35.9 kDa (315aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_004770
- UniProt
- Q9UFF9
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