RCHY1 Protein (AA 1-261) (His tag)

Catalog No. ABIN5853062

Product Details

Target: RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))

Protein Type: Recombinant

Protein Characteristics: AA 1-261

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RCHY1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAATARE DGASGQERGQ RGCEHYDRGC LLKAPCCDKL YTCRLCHDNN EDHQLDRFKV KEVQCINCEK IQHAQQTCEE CSTLFGEYYC DICHLFDKDK KQYHCENCGI CRIGPKEDFF HCLKCNLCLA MNLQGRHKCI ENVSRQNCPI CLEDIHTSRV VAHVLPCGHL LHRTCYEEML KEGYRCPLCM HSALDMTRYW RQLDDEVAQT PMPSEYQNMT VDILCNDCNG RSTVQFHILG MKCKICESYN TAQAGGRRIS LDQQ

Purity: > 85 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M uREA, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))

Alternative Name: RCHY1 (RCHY1 Products)

Synonyms: ARNIP Protein, CHIMP Protein, PIRH2 Protein, PRO1996 Protein, RNF199 Protein, ZNF363 Protein, Pirh2 Protein, Zfp363 Protein, Znf363 Protein, wu:fb37e07 Protein, zgc:101128 Protein, RCHY1 Protein, arnip Protein, chimp Protein, pirh2 Protein, harnip Protein, rnf199 Protein, znf363 Protein, pro1996 Protein, ring finger and CHY zinc finger domain containing 1 Protein, ring finger and CHY zinc finger domain containing 1 L homeolog Protein, RCHY1 Protein, Rchy1 Protein, rchy1 Protein, rchy1.L Protein

Background: RCHY1 has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including TP53, HDAC1 and CDKN1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Recombinant human RCHY1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molecular Weight: 32.5 kDa (284aa)

NCBI Accession: NP_056251

UniProt: Q96PM5