PCBP1 Protein (AA 1-163) (His tag)
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- Target See all PCBP1 Proteins
- PCBP1 (Poly(rC) Binding Protein 1 (PCBP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-163
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PCBP1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMDAGVTE SGLNVTLTIR LLMHGKEVGS IIGKKGESVK RIREESGARI NISEGNCPER IITLTGPTNA IFKAFAMIID KLEEDINSSM TNSTAASRPP VTLRLVVPAT QCGSLIGKGG CKIKEIREST GAQVQVAGDM LPNSTERAIT IAGVPQSVTE CVKQIC
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product PCBP1 Protein
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Poly(rC) Binding Protein 1 (PCBP1) (AA 1-163) protein (His tag)
PCBP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Poly(rC) Binding Protein 1 (PCBP1) (AA 1-356) protein (GST tag)PCBP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Poly(rC) Binding Protein 1 (PCBP1) (AA 181-280) protein (GST tag)PCBP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Poly(rC) Binding Protein 1 (PCBP1) (AA 1-356) protein (Strep Tag)Crystallography grade PCBP1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Poly(rC) Binding Protein 1 (PCBP1) (AA 1-356) protein (Strep Tag)Crystallography grade PCBP1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Poly(rC) Binding Protein 1 (PCBP1) protein (Myc-DYKDDDDK Tag)PCBP1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PCBP1 (Poly(rC) Binding Protein 1 (PCBP1))
- Alternative Name
- PCBP1 (PCBP1 Products)
- Synonyms
- PCBP1 Protein, HNRPE1 Protein, HNRPX Protein, hnRNP-E1 Protein, hnRNP-X Protein, hnRNP E1 Protein, WBP17 Protein, [a]CP-1 Protein, alphaCP-1 Protein, RGD1561319 Protein, poly(rC) binding protein 1 Protein, poly(rC) binding protein 1 L homeolog Protein, poly(rC)-binding protein 1 Protein, PCBP1 Protein, pcbp1.L Protein, LOC100388387 Protein, Pcbp1 Protein
- Background
- PCBP1 appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promotes poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP1 is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. Recombinant human PCBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 19.6 kDa (186aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_006187
- UniProt
- Q15365
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