SPRY4 Protein (AA 1-299) (His tag)
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- Target See all SPRY4 Proteins
- SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-299
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SPRY4 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMEPPIPQ SAPLTPNSVM VQPLLDSRMS HSRLQHPLTI LPIDQVKTSH VENDYIDNPS LALTTGPKRT RGGAPELAPT PARCDQDVTH HWISFSGRPS SVSSSSSTSS DQRLLDHMAP PPVADQASPR AVRIQPKVVH CQPLDLKGPA VPPELDKHFL LCEACGKCKC KECASPRTLP SCWVCNQECL CSAQTLVNYG TCMCLVQGIF YHCTNEDDEG SCADHPCSCS RSNCCARWSF MGALSVVLPC LLCYLPATGC VKLAQRGYDR LRRPGCRCKH TNSVICKAAS GDAKTSRPDK PF
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product SPRY4 Protein
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Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 16-115) protein (GST tag)
SPRY4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 1-299) protein (Strep Tag)Crystallography grade SPRY4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 1-300) protein (Strep Tag)Crystallography grade SPRY4 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.4M urea
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))
- Alternative Name
- SPRY4 (SPRY4 Products)
- Synonyms
- SPRY4 Protein, LOC100218545 Protein, HH17 Protein, A030006O18Rik Protein, sprouty4 Protein, sprouty RTK signaling antagonist 4 Protein, sprouty (Drosophila) homolog 4 Protein, sprouty homolog 4 (Drosophila) Protein, Spry4 Protein, SPRY4 Protein, spry4 Protein
- Background
- SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS (see HRAS, MIM 190020) activation and impairs the formation of active GTP-RAS. Recombinant human SPRY4 protein, fused to His-tag at N-terminus, was expressed in E.coli .
- Molecular Weight
- 34.9 kDa (322aa)
- NCBI Accession
- NP_001120968
- UniProt
- Q9C004
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