G Antigen 2A Protein (GAGE2A) (AA 1-116) (His tag)
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- Target See all G Antigen 2A (GAGE2A) Proteins
- G Antigen 2A (GAGE2A)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-116
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This G Antigen 2A protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMSWRGRS TYRPRPRRYV EPPEMIGPMR PEQFSDEVEP ATPEEGEPAT QRQDPAAAQE GQDEGASAGQ GPKPEAHSQE QGHPQTGCEC EDGPDGQEMD PPNPEEVKTP EEGEKQSQC
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product GAGE2A Protein
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G Antigen 2A (GAGE2A) (AA 1-116) protein (His tag)
GAGE2A Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
G Antigen 2A (GAGE2A) (AA 1-116) protein (Strep Tag)Crystallography grade GAGE2A Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- G Antigen 2A (GAGE2A)
- Alternative Name
- GAGE2A (GAGE2A Products)
- Synonyms
- CT4.2 Protein, GAGE-2 Protein, GAGE-2A Protein, GAGE2 Protein, G antigen 2A Protein, GAGE2A Protein
- Background
- GAGE2A belongs to a multigene family expressed in a large variety of tumors whereas in normal tissues, expression is restricted to germ cells. These genes organized in clustered repeats, have a high degree of predicted sequence identity, but differ by scattered single nucleotide substitution. Their sequences contain either the antigenic peptide YYWPRPRRY or YRPRPRRY which is recognized by cytotoxic T-cells. Recombinant human GAGE2A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 15 kDa (139aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI Accession
- NP_001120684
- UniProt
- Q6NT46
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