MBP Protein (AA 1-197) (His tag)
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- Target See all MBP Proteins
- MBP (Myelin Basic Protein (MBP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-197
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MBP protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMGNHAGK RELNAEKAST NSETNRGESE KKRNLGELSR TTSEDNEVFG EADANQNNGT SSQDTAVTDS KRTADPKNAW QDAHPADPGS RPHLIRLFSR DAPGREDNTF KDRPSESDEL QTIQEDSAAT SESLDVMASQ KRPSQRHGSK YLATASTMDH ARHGFLPRHR DTGILDSIGR FFGGDRGAPK RGSGKVSSEE
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product MBP Protein
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Myelin Basic Protein (MBP) (AA 1-160) protein (GST tag)
MBP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Myelin Basic Protein (MBP) (AA 1-304) protein (His tag)Crystallography grade MBP Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Myelin Basic Protein (MBP) (AA 1-250) protein (His tag)Crystallography grade MBP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol,
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- MBP (Myelin Basic Protein (MBP))
- Alternative Name
- Myelin basic protein (MBP Products)
- Synonyms
- C76307 Protein, Hmbpr Protein, R75289 Protein, golli-mbp Protein, mld Protein, shi Protein, Mbps Protein, cb274 Protein, fj33b11 Protein, mbp Protein, wu:fj33b11 Protein, wu:fq15b02 Protein, zgc:136630 Protein, MGC115135 Protein, myelin basic protein Protein, myelin basic protein a Protein, myelin basic protein L homeolog Protein, MBP Protein, Mbp Protein, mbpa Protein, mbp Protein, mbp.L Protein
- Background
- MBP is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Recombinant human MBP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 23.9 kDa (220aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_001020271
- UniProt
- P02686
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