EIF3I Protein (AA 1-325) (His tag)

Catalog No. ABIN5853326

Product Details

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Protein Type: Recombinant

Protein Characteristics: AA 1-325

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This EIF3I protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMKPILLQ GHERSITQIK YNREGDLLFT VAKDPIVNVW YSVNGERLGT YMGHTGAVWC VDADWDTKHV LTGSADNSCR LWDCETGKQL ALLKTNSAVR TCGFDFGGNI IMFSTDKQMG YQCFVSFFDL RDPSQIDNNE PYMKIPCNDS KITSAVWGPL GECIIAGHES GELNQYSAKS GEVLVNVKEH SRQINDIQLS RDMTMFVTAS KDNTAKLFDS TTLEHQKTFR TERPVNSAAL SPNYDHVVLG GGQEAMDVTT TSTRIGKFEA RFFHLAFEEE FGRVKGHFGP INSVAFHPDG KSYSSGGEDG YVRIHYFDPQ YFEFEFEA

Purity: > 90 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 10 % glycerol 0.4M urea

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Alternative Name: EIF3I (EIF3I Products)

Synonyms: EIF3S2 Protein, PRO2242 Protein, TRIP-1 Protein, TRIP1 Protein, eIF3-beta Protein, eIF3-p36 Protein, 36kDa Protein, D4Ertd632e Protein, Eif3s2 Protein, Trip1 Protein, eIF-3-beta Protein, eIF3 p36 Protein, eif3s2 Protein, hm:zehn0464 Protein, wu:fb62b08 Protein, zgc:56211 Protein, zgc:77823 Protein, CaO19.2967 Protein, eIF-3 39 kDa subunit homolog Protein, eIF3i Protein, eif3-beta Protein, eif3-p36 Protein, eif3i Protein, pro2242 Protein, trip-1 Protein, trip1 Protein, eukaryotic translation initiation factor 3 subunit I Protein, eukaryotic translation initiation factor 3, subunit I Protein, Eukaryotic Initiation Factor Protein, Eukaryotic translation initiation factor 3 subunit I Protein, translation initiation factor eIF3 subunit i Protein, eukaryotic translation initiation factor 3 subunit I L homeolog Protein, EIF3I Protein, Eif3i Protein, eif-3.I Protein, eIF3i Protein, eif3i Protein, TIF34 Protein, eif3i.L Protein

Background: EIF3I is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2: GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AuG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Recombinant human EIF3I protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molecular Weight: 38.9kDa (348aa)

NCBI Accession: NP_003748

UniProt: Q13347

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Ribonucleoprotein Complex Subunit Organization, Synthesis of DNA