Dynamitin Protein (AA 1-401) (His tag)

Catalog No. ABIN5853385

Product Details

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Protein Type: Recombinant

Protein Characteristics: AA 1-401

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Dynamitin protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADPKYA DLPGIARNEP DVYETSDLPE DDQAEFDAEE LTSTSVEHII VNPNAAYDKF KDKRVGTKGL DFSDRIGKTK RTGYESGEYE MLGEGLGVKE TPQQKYQRLL HEVQELTTEV EKIKTTVKES ATEEKLTPVL LAKQLAALKQ QLVASHLEKL LGPDAAINLT DPDGALAKRL LLQLEATKNS KGGSGGKTTG TPPDSSLVTY ELHSRPEQDK FSQAAKVAEL EKRLTELETA VRCDQDAQNP LSAGLQGACL METVELLQAK VSALDLAVLD QVEARLQSVL GKVNEIAKHK ASVEDADTQS KVHQLYETIQ RWSPIASTLP ELVQRLVTIK QLHEQAMQFG QLLTHLDTTQ QMIANSLKDN TTLLTQVQTT MRENLATVEG NFASIDERMK KLGK

Purity: > 90 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl(  pH 8.0) containing 20 % glycerol, 0.15M NaCl, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Alternative Name: DCTN2 (DCTN2 Products)

Synonyms: DCTN2 Protein, dctn2 Protein, dctn50 Protein, dynamitin Protein, rbp50 Protein, CG8269 Protein, DMN Protein, Dmel\\CG8269 Protein, P50 Protein, dDyn Protein, dmn Protein, dyna Protein, l(2)k16109 Protein, l(2)k16218 Protein, p50 Protein, p50/Dmn Protein, p50/dynamitin Protein, 2310042E05Rik Protein, C130077D06Rik Protein, C87049 Protein, DCTN-50 Protein, GMP23-48K Protein, RBP50 Protein, DCTN50 Protein, DYNAMITIN Protein, zgc:63867 Protein, dynactin subunit 2 Protein, dynactin subunit 2 S homeolog Protein, Dynactin 2, p50 subunit Protein, dynactin 2 Protein, dynactin 2 (p50) Protein, dynactin subunit 2 L homeolog Protein, DCTN2 Protein, dctn2.S Protein, DCTN2-p50 Protein, dctn2 Protein, Dctn2 Protein, dctn2.L Protein

Background: DCTN2 is a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Recombinant human DCTN2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 46.6kDa (424aa), confirmed by MALDI-TOF

NCBI Accession: NP_001248342

UniProt: Q13561

Pathways: M Phase, Ribonucleoprotein Complex Subunit Organization