SHFM1 Protein (AA 1-70) (His tag)
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- Target See all SHFM1 Proteins
- SHFM1 (Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-70
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SHFM1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMSEKKQP VDLGLLEEDD EFEEFPAEDW AGLDEDEDAH VWEDNWDDDN VEDDFSNQLR AELEKHGYKM ETS
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product SHFM1 Protein
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Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1) (AA 1-70) protein (His tag)
SHFM1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1) (AA 1-70) protein (GST tag)SHFM1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1) (AA 1-70) protein (His tag)Crystallography grade SHFM1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1) (AA 1-128) protein (His tag)Crystallography grade SHFM1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.1M NaCl
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- SHFM1 (Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1))
- Alternative Name
- SHFM1 (SHFM1 Products)
- Synonyms
- DSS1 Protein, ECD Protein, SEM1 Protein, SHFD1 Protein, SHSF1 Protein, Shfdg1 Protein, Shfg Protein, Dss1 Protein, SHFM1 Protein, dss1 Protein, ecd Protein, sem1 Protein, shfd1 Protein, shfdg1 Protein, shfm1 Protein, shfm1.S Protein, shsf1 Protein, wu:fb55c02 Protein, zgc:56203 Protein, zgc:85901 Protein, SEM1, 26S proteasome complex subunit Protein, SEM1, 26S proteasome complex subunit S homeolog Protein, SEM1 Protein, Sem1 Protein, sem1.S Protein, sem1 Protein
- Background
- SHFM1, as known as 26S proteasome complex subunit DSS1, has been proposed to be a candidate gene for the autosomal dominant form of the heterogeneous limb developmental disorder split hand/split foot malformation type 1. In addition, it has been shown to directly interact with BRCA2. It also may play a role in the completion of the cell cycle. Recombinant human SHFM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 10.7kDa (93aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI Accession
- NP_006295
- UniProt
- P60896
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