MAP1D Protein (AA 20-335) (His tag)
-
- Target See all MAP1D (METAP1D) Proteins
- MAP1D (METAP1D) (Methionyl Aminopeptidase Type 1D (Mitochondrial) (METAP1D))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-335
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MAP1D protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSSSPLNHI YLHKQSSSQQ RRNFFFRRQR DISHSIVLPA AVSSAHPVPK HIKKPDYVTT GIVPDWGDSI EVKNEDQIQG LHQACQLARH VLLLAGKSLK VDMTTEEIDA LVHREIISHN AYPSPLGYGG FPKSVCTSVN NVLCHGIPDS RPLQDGDIIN IDVTVYYNGY HGDTSETFLV GNVDECGKKL VEVARRCRDE AIAACRAGAP FSVIGNTISH ITHQNGFQVC PHFVGHGIGS YFHGHPEIWH HANDSDLPME EGMAFTIEPI ITEGSPEFKV LEDAWTVVSL DNQRSAQFEH TVLITSRGAQ ILTKLPHEA
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product METAP1D Protein
-
Methionyl Aminopeptidase Type 1D (Mitochondrial) (METAP1D) (AA 20-335) protein (His tag)
METAP1D Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Methionyl Aminopeptidase Type 1D (Mitochondrial) (METAP1D) (AA 1-335) protein (GST tag)METAP1D Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Methionyl Aminopeptidase Type 1D (Mitochondrial) (METAP1D) (AA 20-335) protein (His tag)Crystallography grade METAP1D Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Methionyl Aminopeptidase Type 1D (Mitochondrial) (METAP1D) (AA 20-335) protein (His tag)Crystallography grade METAP1D Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 20 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- MAP1D (METAP1D) (Methionyl Aminopeptidase Type 1D (Mitochondrial) (METAP1D))
- Alternative Name
- METAP1D (METAP1D Products)
- Synonyms
- MAP 1D Protein, MAP1D Protein, MetAP 1D Protein, Metap1l Protein, 2310066F24Rik Protein, 3110033D18Rik Protein, AV117938 Protein, Map1d Protein, Metapl1 Protein, map1d Protein, zgc:110461 Protein, methionyl aminopeptidase type 1D, mitochondrial Protein, methionyl aminopeptidase type 1D (mitochondrial) Protein, METAP1D Protein, Metap1d Protein, metap1d Protein
- Background
- Methionine aminopeptidase 1D, mitochondrial precursor, also known as METAP1D, is a 335 amino acid mitochondrial protein that belongs to the peptidase M24A family. It is overexpressed in colon cancer cell lines, suggesting a role in tumorigenesis. METAP1D has also been found to remove methionine from the N-terminus of nascent proteins. METAP1D binds two cobalt ions per subunit and is encoded by a gene that maps to human chromosome 2q31.1. Recombinant human METAP1D protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 37.4 kDa (339aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_954697
- UniProt
- Q6UB28
-
