MLF1IP Protein (AA 147-418) (His tag)
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- Target See all MLF1IP Proteins
- MLF1IP (MLF1 Interacting Protein (MLF1IP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 147-418
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MLF1IP protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSTRRKVKS AEKISTQRHE VIRTTASSEL SEKPAESVTS KKTGPLSAQP SVEKENLAIE SQSKTQKKGK ISHDKRKKSR SKAIGSDTSD IVHIWCPEGM KTSDIKELNI VLPEFEKTHL EHQQRIESKV CKAAIATFYV NVKEQFIKML KESQMLTNLK RKNAKMISDI EKKRQRMIEV QDELLRLEPQ LKQLQTKYDE LKERKSSLRN AAYFLSNLKQ LYQDYSDVQA QEPNVKETYD SSSLPALLFK ARTLLGAESH LRNINHQLEK LLDQG
- Purity
- > 80 % by SDS - PAGE
- Top Product
- Discover our top product MLF1IP Protein
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MLF1 Interacting Protein (MLF1IP) (AA 1-176) protein (GST tag)
MLF1IP Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
MLF1 Interacting Protein (MLF1IP) (AA 1-418) protein (Strep Tag)Crystallography grade MLF1IP Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
MLF1 Interacting Protein (MLF1IP) (AA 1-410) protein (Strep Tag)Crystallography grade MLF1IP Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.4M urea
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- MLF1IP (MLF1 Interacting Protein (MLF1IP))
- Alternative Name
- MLF1IP (MLF1IP Products)
- Synonyms
- MGC147270 Protein, CENP50 Protein, CENPU Protein, CENPU50 Protein, KLIP1 Protein, PBIP1 Protein, CENP-U Protein, CENP-50 Protein, 1700029A22Rik Protein, centromere protein U Protein, MLF1 interacting protein Protein, cenpu Protein, MLF1IP Protein, CENPU Protein, Cenpu Protein
- Background
- MLF1IP, as known as centromere, is a specialized chromatin domain, present throughout the cell cycle that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. It plays an important role in the correct PLK1 localization to the mitotic kinetochores. It is a scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. It also involved in transcriptional repression. Recombinant human MLF1IP protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 33.7kDa (295aa)
- NCBI Accession
- NP_078905
- UniProt
- Q71F23
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