BRK1 Protein (AA 1-75) (His tag)
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- Target See all BRK1 Proteins
- BRK1 (BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-75
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BRK1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMAGQEDP VQREIHQDWA NREYIEIITS SIKKIADFLN SFDMSCRSRL ATLNEKLTAL ERRIEYIEAR VTKGETLT
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product BRK1 Protein
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BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1) (AA 1-75) protein (His tag)
BRK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1) (AA 1-75) protein (GST tag)BRK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1) (AA 2-75) protein (His tag)Crystallography grade BRK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1) (AA 2-75) protein (His tag)Crystallography grade BRK1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1) protein (Myc-DYKDDDDK Tag)BRK1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- BRK1 (BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1))
- Alternative Name
- BRK1 (BRK1 Products)
- Synonyms
- brk1 Protein, C22H3orf10 Protein, brick1 Protein, c3orf10 Protein, C3orf10 Protein, MDS027 Protein, hHBrk1 Protein, 6720456B07Rik Protein, AW011779 Protein, ATBRK1 Protein, BRICK1 Protein, HSPC300 Protein, T9I22.8 Protein, T9I22_8 Protein, BRICK1, SCAR/WAVE actin-nucleating complex subunit Protein, BRICK1, SCAR/WAVE actin nucleating complex subunit Protein, brick 1 Protein, BRICK1, SCAR/WAVE actin-nucleating complex subunit S homeolog Protein, BRICK1 Protein, brk1 Protein, BRK1 Protein, Brk1 Protein, brk1.S Protein
- Background
- BRK1 involved in regulation of actin and microtubule organization. This protein is a part of a WAVE complex that activates the Arp2/3 complex. It belongs to the BRK1 family. Recombinant human BRK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 11 kDa (98aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_060932
- UniProt
- Q8WUW1
- Pathways
- RTK Signaling
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