CD73 Protein (AA 27-252) (His tag)
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- Target See all CD73 (NT5E) Proteins
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-252
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CD73 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MWELTILHTN DVHSRLEQTS EDSSKCVNAS RCMGGVARLF TKVQQIRRAE PNVLLLDAGD QYQGTIWFTV YKGAEVAHFM NALRYDAMAL GNHEFDNGVE GLIEPLLKEA KFPILSANIK AKGPLASQIS GLYLPYKVLP VGDEVVGIVG YTSKETPFLS NPGTNLVFED EITALQPEVD KLKTLNVNKI IALGHSGFEM DKLIAQKVRG VDVVVGGHSN TFLYTGN
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product NT5E Protein
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5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-549) protein (His tag)
NT5E Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-547) protein (His tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-547) protein (Fc Tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-554) protein (His tag)NT5E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (His tag)NT5E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (Fc Tag)NT5E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) protein (His tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) (Active) protein (His tag,AVI tag,Biotin)NT5E Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 34-214) protein (His tag)NT5E Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 28-264) protein (GST tag)NT5E Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- Alternative Name
- NT5E (NT5E Products)
- Synonyms
- CALJA Protein, CD73 Protein, E5NT Protein, NT Protein, NT5 Protein, NTE Protein, eN Protein, eNT Protein, zgc:63784 Protein, NT5E Protein, MGC114869 Protein, PSPTO2277 Protein, 2210401F01Rik Protein, AI447961 Protein, Nt5 Protein, 5'-nucleotidase ecto Protein, 5'-nucleotidase, ecto (CD73) Protein, 5'-nucleotidase Protein, 5' nucleotidase, ecto Protein, NT5E Protein, nt5e Protein, nt5e.S Protein, PSPTO_2277 Protein, CBU_1233 Protein, MCA1322 Protein, Nt5e Protein
- Background
- NT5E is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. This protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human NT5E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 27.0 kDa (247aa)
- NCBI Accession
- NP_002517
- UniProt
- P21589
- Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
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