POLR2F Protein (AA 1-127) (His tag)
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- Target See all POLR2F Proteins
- POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-127
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This POLR2F protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMSDNEDN FDGDDFDDVE EDEGLDDLEN AEEEGQENVE ILPSGERPQA NQKRITTPYM TKYERARVLG TRALQIAMCA PVMVELEGET DPLLIAMKEL KARKIPIIIR RYLPDGSYED WGVDELIITD
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product POLR2F Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))
- Alternative Name
- POLR2F (POLR2F Products)
- Synonyms
- HRBP14.4 Protein, POLRF Protein, RPABC2 Protein, RPB14.4 Protein, RPB6 Protein, 1810060D16Rik Protein, RNA polymerase II subunit F Protein, polymerase (RNA) II (DNA directed) polypeptide F Protein, POLR2F Protein, Polr2f Protein
- Background
- POLR2F is the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Recombinant human POLR2F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 16.9kDa (150aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI Accession
- NP_068809
- UniProt
- P61218
- Pathways
- Regulatory RNA Pathways
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