REV1 Protein (AA 51-256) (His tag)
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- Target See all REV1 Proteins
- REV1 (REV1, Polymerase (DNA Directed) (REV1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 51-256
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This REV1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGVAIYVNGY TDPSAEELRK LMMLHGGQYH VYYSRSKTTH IIATNLPNAK IKELKGEKVI RPEWIVESIK AGRLLSYIPY QLYTKQSSVQ KGLSFNPVCR PEDPLPGPSN IAKQLNNRVN HIVKKIETEN EVKVNGMNSW NEEDENNDFS FVDLEQTSPG RKQNGIPHPR GSTAIFNGHT PSSNGALKTQ DCLVPMVNSV ASRLSPA
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product REV1 Protein
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REV1, Polymerase (DNA Directed) (REV1) (AA 1097-1194) protein (GST tag)
REV1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
REV1, Polymerase (DNA Directed) (REV1) (AA 1-1251) protein (His tag)Crystallography grade REV1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
REV1, Polymerase (DNA Directed) (REV1) (AA 1-1249) protein (His tag)Crystallography grade REV1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M urea, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- REV1 (REV1, Polymerase (DNA Directed) (REV1))
- Alternative Name
- REV1 (REV1 Products)
- Synonyms
- ARABIDOPSIS THALIANA HOMOLOG OF REVERSIONLESS 1 Protein, ATREV1 Protein, K23L20.9 Protein, K23L20_9 Protein, 1110027I23Rik Protein, AU022044 Protein, Rev1l Protein, REV1L Protein, DNA-directed DNA polymerase Protein, HIG1 hypoxia inducible domain family member 1C Protein, REV1, DNA directed polymerase Protein, REV1 Protein, HIGD1C Protein, Rev1 Protein
- Background
- REV1 contains a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found. Recombinant human REV1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 25.2 kDa (227aa)
- NCBI Accession
- NP_057400
- UniProt
- Q9UBZ9
- Pathways
- DNA Damage Repair, Regulation of Actin Filament Polymerization, Production of Molecular Mediator of Immune Response
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