CYB5R2 Protein (AA 1-276) (His tag)
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- Target See all CYB5R2 Proteins
- CYB5R2 (Cytochrome B5 Reductase 2 (CYB5R2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-276
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CYB5R2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMNSRRRE PITLQDPEAK YPLPLIEKEK ISHNTRRFRF GLPSPDHVLG LPVGNYVQLL AKIDNELVVR AYTPVSSDDD RGFVDLIIKI YFKNVHPQYP EGGKMTQYLE NMKIGETIFF RGPRGRLFYH GPGNLGIRPD QTSEPKKTLA DHLGMIAGGT GITPMLQLIR HITKDPSDRT RMSLIFANQT EEDILVRKEL EEIARTHPDQ FNLWYTLDRP PIGWKYSSGF VTADMIKEHL PPPAKSTLIL VCGPPPLIQT AAHPNLEKLG YTQDMIFTY
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product CYB5R2 Protein
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Cytochrome B5 Reductase 2 (CYB5R2) (AA 1-276) protein (His tag)
CYB5R2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Cytochrome B5 Reductase 2 (CYB5R2) (AA 1-237) protein (GST tag)CYB5R2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Cytochrome B5 Reductase 2 (CYB5R2) (AA 1-276) protein (Strep Tag)Crystallography grade CYB5R2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Cytochrome B5 Reductase 2 (CYB5R2) (AA 1-276) protein (Strep Tag)Crystallography grade CYB5R2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Cytochrome B5 Reductase 2 (CYB5R2) protein (Myc-DYKDDDDK Tag)CYB5R2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CYB5R2 (Cytochrome B5 Reductase 2 (CYB5R2))
- Alternative Name
- CYB5R2 (CYB5R2 Products)
- Synonyms
- B5R.2 Protein, D630003K02Rik Protein, b5R.2 Protein, RGD1308421 Protein, b5r Protein, im:7147295 Protein, zgc:153291 Protein, cytochrome b5 reductase 2 S homeolog Protein, cytochrome b5 reductase 2 Protein, cyb5r2.S Protein, CYB5R2 Protein, cyb5r2 Protein, Cyb5r2 Protein
- Background
- Cytochrome b5 reductase 2, also known as CYB5R2, is involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. This protein is responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1). Recombinant human CYB5R2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 33.8 kDa (299aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_057313
- UniProt
- Q6BCY4
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