POLR1C Protein (AA 1-349) (His tag)
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- Target See all POLR1C Proteins
- POLR1C (Polymerase (RNA) I Polypeptide C, 30kDa (POLR1C))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-349
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This POLR1C protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMAASQAV EEMRSRVVLG EFGVRNVHTT DFPGNYSGYD DAWDQDRFEK NFRVDVVHMD ENSLEFDMVG IDAAIANAFR RILLAEVPTM AVEKVLVYNN TSIVQDEILA HRLGLIPIHA DPRLFEYRNQ GDEEGTEIDT LQFRLQVRCT RNPHAAKDSS DPNELYVNHK VYTRHMTWIP LGNQADLFPE GTIRPVHDDI LIAQLRPGQE IDLLMHCVKG IGKDHAKFSP VATASYRLLP DITLLEPVEG EAAEELSRCF SPGVIEVQEV QGKKVARVAN PRLDTFSREI FRNEKLKKVV RLARVRDHYI FSVESTGVLP PDVLVSEAIK VLMGKCRRFL DELDAVQMD
- Purity
- > 80 % by SDS - PAGE
- Top Product
- Discover our top product POLR1C Protein
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Polymerase (RNA) I Polypeptide C, 30kDa (POLR1C) (AA 1-346) protein (His tag)
POLR1C Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Polymerase (RNA) I Polypeptide C, 30kDa (POLR1C) (AA 1-346) protein (GST tag)POLR1C Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (RNA) I Polypeptide C, 30kDa (POLR1C) (AA 2-346) protein (His tag)Crystallography grade POLR1C Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Polymerase (RNA) I Polypeptide C, 30kDa (POLR1C) (AA 2-346) protein (His tag)Crystallography grade POLR1C Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Polymerase (RNA) I Polypeptide C, 30kDa (POLR1C) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)POLR1C Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- POLR1C (Polymerase (RNA) I Polypeptide C, 30kDa (POLR1C))
- Alternative Name
- POLR1C (POLR1C Products)
- Synonyms
- zgc:65973 Protein, rpa39 Protein, rpa40 Protein, rpa5 Protein, rpac1 Protein, POLR1C Protein, 40kDa Protein, AA409007 Protein, AA959927 Protein, AL024089 Protein, Polr1e Protein, RPA40 Protein, Rpo1-1 Protein, Ac2-127 Protein, RPA39 Protein, RPA5 Protein, RPAC1 Protein, TCS3 Protein, polymerase (RNA) I polypeptide C Protein, RNA polymerase I subunit C Protein, polymerase (RNA) I polypeptide C L homeolog Protein, polymerase (RNA) I polypeptide C, 30kDa Protein, polr1c Protein, POLR1C Protein, polr1c.L Protein, Polr1c Protein
- Background
- POLR1C is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Defects in this gene have been associated with Treacher Collins syndrome (TCS). Recombinant human POLR1C protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 41.6 kDa (369aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_976035
- UniProt
- O15160
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