HCV E2 Protein (AA 482-671) (His tag)

Catalog No. ABIN5853806

Product Details

Target: HCV E2 (Hepatitis C Virus Envelope Protein E2 (HCV E2))

Protein Type: Recombinant

Protein Characteristics: AA 482-671

Origin: Hepatitis C Virus (HCV)

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This HCV E2 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSERPY CWHYPPRPCG IVPAKSVCGP VYCFTPSPVV VGTTDRSGAP TYSWGANDTD VFVLNNTRPP LGNWFGCTWM NSTGFTKVCG APPCVIGGVG NNTLLCPTDC FRKHPEATYS RCGSGPWITP RCMVDYPYRL WHYPCTINYT IFKVRMYVGG VEHRLEAACN WTRGERCDLE DRDRSELSPL LLSTTQ

Purity: > 80 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M urea, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: HCV E2 (Hepatitis C Virus Envelope Protein E2 (HCV E2))

Alternative Name: HCV-E2 (HCV E2 Products)

Target Type: Viral Protein

Background: E1 and E2 glycoproteins form a heterodimer that is involved in virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. E1/E2 heterodimer binds to human LDLR, CD81 and SCARB1/SR-BI receptors, but this binding is not sufficient for infection, some additional liver specific cofactors may be needed. The fusion function may possibly be carried by E1. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. Recombinant HCV(Hepatitis C Virus) E2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molecular Weight: 25.4 kDa (226aa)

NCBI Accession: NP_671491