PPA2 Protein (AA 33-334) (His tag)
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- Target See all PPA2 Proteins
- PPA2 (Pyrophosphatase 2 (PPA2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 33-334
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PPA2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSALYHTEE RGQPCSQNYR LFFKNVTGHY ISPFHDIPLK VNSKEENGIP MKKARNDEYE NLFNMIVEIP RWTNAKMEIA TKEPMNPIKQ YVKDGKLRYV ANIFPYKGYI WNYGTLPQTW EDPHEKDKST NCFGDNDPID VCEIGSKILS CGEVIHVKIL GILALIDEGE TDWKLIAINA NDPEASKFHD IDDVKKFKPG YLEATLNWFR LYKVPDGKPE NQFAFNGEFK NKAFALEVIK STHQCWKALL MKKCNGGAIN CTNVQISDSP FRCTQEEARS LVESVSSSPN KESNEEEQVW HFLGK
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product PPA2 Protein
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Pyrophosphatase 2 (PPA2) (AA 33-334) protein (His tag)
PPA2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Pyrophosphatase 2 (PPA2) (AA 1-334) protein (GST tag)PPA2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Pyrophosphatase 2 (PPA2) (AA 1-90) protein (GST tag)PPA2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Pyrophosphatase 2 (PPA2) (AA 33-334) protein (His tag)Crystallography grade PPA2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Pyrophosphatase 2 (PPA2) (AA 28-330) protein (His tag)Crystallography grade PPA2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Pyrophosphatase 2 (PPA2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PPA2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PPA2 (Pyrophosphatase 2 (PPA2))
- Alternative Name
- PPA2 (PPA2 Products)
- Synonyms
- SID6-306 Protein, RGD1307965 Protein, 1110013G13Rik Protein, Sid6306 Protein, AtPPa2 Protein, T30D6.26 Protein, T30D6_26 Protein, pyrophosphorylase 2 Protein, zgc:77715 Protein, ppa2 Protein, ppa2.S Protein, pyrophosphatase (inorganic) 2 Protein, pyrophosphorylase 2 Protein, pyrophosphatase (inorganic) 2 L homeolog Protein, PPA2 Protein, Ppa2 Protein, PPa2 Protein, ppa2 Protein, ppa2.L Protein
- Background
- PPA2 is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Recombinant human PPA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 37.1 kDa (325aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_789845
- UniProt
- Q9H2U2
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