EIF4EBP3 Protein (AA 1-100) (His tag)
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- Target See all EIF4EBP3 Proteins
- EIF4EBP3 (Eukaryotic Translation Initiation Factor 4E Binding Protein 3 (EIF4EBP3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-100
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This EIF4EBP3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMSTSTSC PIPGGRDQLP DCYSTTPGGT LYATTPGGTR IIYDRKFLLE CKNSPIARTP PCCLPQIPGV TTPPTAPLSK LEELKEQETE EEIPDDAQFE MDI
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product EIF4EBP3 Protein
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Eukaryotic Translation Initiation Factor 4E Binding Protein 3 (EIF4EBP3) (AA 1-100) protein (His tag)
EIF4EBP3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Eukaryotic Translation Initiation Factor 4E Binding Protein 3 (EIF4EBP3) (AA 1-100) protein (GST tag)EIF4EBP3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Eukaryotic Translation Initiation Factor 4E Binding Protein 3 (EIF4EBP3) (AA 1-100) protein (Strep Tag)Crystallography grade EIF4EBP3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Eukaryotic Translation Initiation Factor 4E Binding Protein 3 (EIF4EBP3) (AA 1-101) protein (Strep Tag)Crystallography grade EIF4EBP3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Eukaryotic Translation Initiation Factor 4E Binding Protein 3 (EIF4EBP3) (AA 1-100), (full length) protein (His-SUMO Tag)EIF4EBP3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Eukaryotic Translation Initiation Factor 4E Binding Protein 3 (EIF4EBP3) protein (Myc-DYKDDDDK Tag)EIF4EBP3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- EIF4EBP3 (Eukaryotic Translation Initiation Factor 4E Binding Protein 3 (EIF4EBP3))
- Alternative Name
- EIF4EBP3 (EIF4EBP3 Products)
- Synonyms
- zgc:103720 Protein, 4E-BP3 Protein, 4EBP3 Protein, 4e-bp3 Protein, eukaryotic translation initiation factor 4E binding protein 3 Protein, eif4ebp3 Protein, Eif4ebp3 Protein, EIF4EBP3 Protein
- Background
- Eukaryotic translation initiation factor 4E-binding protein 3, also known as EIF4EBP 3, is a member of EIF4EBP family which derives its name from proteins that bind to eukaryotic initiation factor 4E and that prevent its assembly into EIF4F. The EIF4F subunit EIF4E interacts directly with the mRNA 5' cap structure. Co-transcription of this gene and the neighboring upstream gene (MASK) generates a transcript (MASK-BP3) which encodes a fusion protein comprised of the MASK protein sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. Recombinant human EIF4EBP3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 13.3 kDa (123aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_003723
- UniProt
- O60516
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