RNGTT Protein (AA 1-597) (His tag)
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- Target See all RNGTT Proteins
- RNGTT (RNA Guanylyltransferase and 5'-Phosphatase (RNGTT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-597
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RNGTT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMAHNKIP PRWLNCPRRG QPVAGRFLPL KTMLGPRYDS QVAEENRFHP SMLSNYLKSL KVKMGLLVDL TNTSRFYDRN DIEKEGIKYI KLQCKGHGEC PTTENTETFI RLCERFNERN PPELIGVHCT HGFNRTGFLI CAFLVEKMDW SIEAAVATFA QARPPGIYKG DYLKELFRRY GDIEEAPPPP LLPDWCFEDD EDEDEDEDGK KESEPGSSAS FGKRRKERLK LGAIFLEGVT VKGVTQVTTQ PKLGEVQQKC HQFCGWEGSG FPGAQPVSMD KQNIKLLDLK PYKVSWKADG TRYMMLIDGT NEVFMIDRDN SVFHVSNLEF PFRKDLRMHL SNTLLDGEMI IDRVNGQAVP RYLIYDIIKF NSQPVGDCDF NVRLQCIERE IISPRHEKMK TGLIDKTQEP FSVRNKPFFD ICTSRKLLEG NFAKEVSHEM DGLIFQPTGK YKPGRCDDIL KWKPPSLNSV DFRLKITRMG GEGLLPQNVG LLYVGGYERP FAQIKVTKEL KQYDNKIIEC KFENNSWVFM RQRTDKSFPN AYNTAMAVCN SISNPVTKEM LFEFIDRCTA ASQGQKRKHH LDPDTELMPP PPPKRPRPLT
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product RNGTT Protein
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RNA Guanylyltransferase and 5'-Phosphatase (RNGTT) (AA 1-597) protein (GST tag)
RNGTT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
RNA Guanylyltransferase and 5'-Phosphatase (RNGTT) (AA 1-597) protein (Strep Tag)Crystallography grade RNGTT Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
RNA Guanylyltransferase and 5'-Phosphatase (RNGTT) (AA 1-597) protein (Strep Tag)Crystallography grade RNGTT Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
RNA Guanylyltransferase and 5'-Phosphatase (RNGTT) protein (Myc-DYKDDDDK Tag)RNGTT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 7.5) containing 0.2M NaCl, 40 % glycerol, 2 mM DTT, 0.1 mM PMSF
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- RNGTT (RNA Guanylyltransferase and 5'-Phosphatase (RNGTT))
- Alternative Name
- RNGTT (RNGTT Products)
- Synonyms
- xcap1b Protein, MGC75787 Protein, CAP1A Protein, HCE Protein, HCE1 Protein, hCAP Protein, AU020997 Protein, zgc:76886 Protein, RNA guanylyltransferase and 5'-phosphatase Protein, mRNA-capping enzyme Protein, rngtt.S Protein, rngtt Protein, RNGTT Protein, LOC100439097 Protein, Rngtt Protein
- Background
- RNGTT is a bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. It catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Recombinant human RNGTT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 70.9 kDa (620aa)
- NCBI Accession
- NP_003791
- UniProt
- O60942
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