NMT1 Protein (AA 1-496) (His tag)
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- Target See all NMT1 Proteins
- NMT1 (N-Myristoyltransferase 1 (NMT1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-496
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NMT1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMADESET AVKPPAPPLP QMMEGNGNGH EHCSDCENEE DNSYNRGGLS PANDTGAKKK KKKQKKKKEK GSETDSAQDQ PVKMNSLPAE RIQEIQKAIE LFSVGQGPAK TMEEASKRSY QFWDTQPVPK LGEVVNTHGP VEPDKDNIRQ EPYTLPQGFT WDALDLGDRG VLKELYTLLN ENYVEDDDNM FRFDYSPEFL LWALRPPGWL PQWHCGVRVV SSRKLVGFIS AIPANIHIYD TEKKMVEINF LCVHKKLRSK RVAPVLIREI TRRVHLEGIF QAVYTAGVVL PKPVGTCRYW HRSLNPRKLI EVKFSHLSRN MTMQRTMKLY RLPETPKTAG LRPMETKDIP VVHQLLTRYL KQFHLTPVMS QEEVEHWFYP QENIIDTFVV ENANGEVTDF LSFYTLPSTI MNHPTHKSLK AAYSFYNVHT QTPLLDLMSD ALVLAKMKGF DVFNALDLME NKTFLEKLKF GIGDGNLQYY LYNWKCPSMG AEKVGLVLQ
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product NMT1 Protein
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N-Myristoyltransferase 1 (NMT1) (AA 1-496) protein (GST tag)
NMT1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
N-Myristoyltransferase 1 (NMT1) (AA 1-496) protein (Strep Tag)Crystallography grade NMT1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
N-Myristoyltransferase 1 (NMT1) (AA 1-496) protein (Strep Tag)Crystallography grade NMT1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
N-Myristoyltransferase 1 (NMT1) protein (Myc-DYKDDDDK Tag)NMT1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In Phosphate buffered saline ( pH 7.4)
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- NMT1 (N-Myristoyltransferase 1 (NMT1))
- Alternative Name
- NMT1 (NMT1 Products)
- Synonyms
- NMT Protein, im:2601337 Protein, nmt1 Protein, wu:fc15d01 Protein, wu:fc18a04 Protein, zgc:110714 Protein, MGC145283 Protein, AW536594 Protein, ARABIDOPSIS THALIANA MYRISTOYL-COA:PROTEIN N-MYRISTOYLTRANSFERASE Protein, ATNMT1 Protein, MHM17.15 Protein, MHM17_15 Protein, N-MYRISTOYLTRANSFERASE 1 Protein, myristoyl-CoA:protein N-myristoyltransferase Protein, N-myristoyltransferase 1 Protein, N-myristoyltransferase 1a Protein, N-myristoyltransferase 1 L homeolog Protein, N-myristoyltransferase 1 (predicted) Protein, myristoyl-CoA:protein N-myristoyltransferase Protein, NMT1 Protein, nmt1a Protein, nmt1.L Protein, nmt1 Protein, SPBC2G2.11 Protein, Nmt1 Protein
- Background
- Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO. Recombinant human NMT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 59.2 kDa (519 aa)
- NCBI Accession
- NP_066565
- UniProt
- P30419
- Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling
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