MRPS28 Protein (AA 72-187) (His tag)
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- Target See all MRPS28 Proteins
- MRPS28 (Mitochondrial Ribosomal Protein S28 (MRPS28))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 72-187
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MRPS28 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSGSPKNVE SFASMLRHSP LTQMGPAKDK LVIGRIFHIV ENDLYIDFGG KFHCVCRRPE VDGEKYQKGT RVRLRLLDLE LTSRFLGATT DTTVLEANAV LLGIQESKDS RSKEEHHEK
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product MRPS28 Protein
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Mitochondrial Ribosomal Protein S28 (MRPS28) (AA 72-187) protein (His tag)
MRPS28 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Mitochondrial Ribosomal Protein S28 (MRPS28) (AA 1-187) protein (GST tag)MRPS28 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Mitochondrial Ribosomal Protein S28 (MRPS28) (AA 71-186) protein (His tag)Crystallography grade MRPS28 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Mitochondrial Ribosomal Protein S28 (MRPS28) protein (Myc-DYKDDDDK Tag)MRPS28 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Phosphate buffer saline ( pH 8.0) containing 20 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- MRPS28 (Mitochondrial Ribosomal Protein S28 (MRPS28))
- Alternative Name
- MRPS28 (MRPS28 Products)
- Synonyms
- LRRGT00031 Protein, Mrps28l Protein, MRP-S28 Protein, MRP-S35 Protein, MRPS35 Protein, 1500012D08Rik Protein, AW061224 Protein, mitochondrial ribosomal protein S28 Protein, Mrps28 Protein, MRPS28 Protein
- Background
- Mammalian mitochondrial ribosomal proteins help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature. Recombinant human MRPS28 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 15.5 kDa (193aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_054737
- UniProt
- Q9Y2Q9
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