THOC7 Protein (AA 1-204) (His tag)
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- Target See all THOC7 Proteins
- THOC7 (THO Complex 7 (THOC7))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-204
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This THOC7 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMGAVTDD EVIRKRLLID GDGAGDDRRI NLLVKSFIKW CNSGSQEEGY SQYQRMLSTL SQCEFSMGKT LLVYDMNLRE MENYEKIYKE IECSIAGAHE KIAECKKQIL QAKRIRKNRQ EYDALAKVIQ HHPDRHETLK ELEALGKELE HLSHIKESVE DKLELRRKQF HVLLSTIHEL QQTLENDEKL SEVEEAQEAS METDPKP
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product THOC7 Protein
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THO Complex 7 (THOC7) (AA 1-204) protein (His tag)
THOC7 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
THO Complex 7 (THOC7) (AA 1-204) protein (GST tag)THOC7 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
THO Complex 7 (THOC7) (AA 2-204) protein (His tag)Crystallography grade THOC7 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
THO Complex 7 (THOC7) (AA 2-204) protein (His tag)Crystallography grade THOC7 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
THO Complex 7 (THOC7) protein (Myc-DYKDDDDK Tag)THOC7 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate buffered saline ( pH 7.4) containing, 50 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- THOC7 (THO Complex 7 (THOC7))
- Alternative Name
- THOC7 (THOC7 Products)
- Synonyms
- RGD1305475 Protein, NIF3L1BP1 Protein, fSAP24 Protein, 1500006O09Rik Protein, 9230101K24Rik Protein, Nif3l1bp1 Protein, THO complex 7 Protein, Thoc7 Protein, THOC7 Protein
- Background
- THOC7 is required for efficient export of polyadenylated RNA. This protein acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Recombinant human THOC7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 26.1kDa (227aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_079351
- UniProt
- Q6I9Y2
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