ATP6V1F Protein (AA 1-119) (His tag)
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- Target See all ATP6V1F Proteins
- ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-119
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ATP6V1F protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMAGRGKL IAVIGDEDTV TGFLLGGIGE LNKNRHPNFL VVEKDTTINE IEDTFRQFLN RDDIGIILIN QYIAEMVRHA LDAHQQSIPA VLEIPSKEHP YDAAKDSILR RARGMFTAED LR
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product ATP6V1F Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate buffered saline ( pH 7.4) containing, 50 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))
- Alternative Name
- ATP6V1F (ATP6V1F Products)
- Synonyms
- Atp6s14 Protein, Vma7 Protein, atp6s14 Protein, atp6v1f Protein, vatf Protein, ATP6S14 Protein, VATF Protein, 1110004G16Rik Protein, ATPase H+ transporting V1 subunit F Protein, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F S homeolog Protein, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F L homeolog Protein, ATPase, H+ transporting, lysosomal V1 subunit F Protein, Atp6v1f Protein, atp6v1f.S Protein, ATP6V1F Protein, atp6v1f.L Protein
- Background
- ATP6V1F is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. Recombinant human ATP6V1F, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 15.8kDa (142aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_004222
- UniProt
- Q16864
- Pathways
- Transition Metal Ion Homeostasis, Proton Transport
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