SPI1 Protein (AA 1-271) (His tag)

Catalog No. ABIN5854082

Product Details

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Protein Type: Recombinant

Protein Characteristics: AA 1-271

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SPI1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLQACKM EGFPLVPPQP SEDLVPYDTD LYQRQTHEYY PYLSSDGESH SDHYWDFHPH HVHSEFESFA ENNFTELQSV QPPQLQQLYR HMELEQMHVL DTPMVPPHPS LGHQVSYLPR MCLQYPSLSP AQPSSDEEEG ERQSPPLEVS DGEADGLEPG PGLLPGETGS KKKIRLYQFL LDLLRSGDMK DSIWWVDKDK GTFQFSSKHK EALAHRWGIQ KGNRKKMTYQ KMARALRNYG KTGEVKKVKK KLTYQFSGEV LGRGGLAERR HPPH

Purity: > 85 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl(  pH 8.0) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: SPI1 (SPI1 Products)

Synonyms: OF Protein, PU.1 Protein, SFPI1 Protein, SPI-1 Protein, SPI-A Protein, Dis-1 Protein, Dis1 Protein, Sfpi-1 Protein, Sfpi1 Protein, Spi-1 Protein, Tcfpu1 Protein, Tfpu.1 Protein, Pu.1 Protein, cb1086 Protein, pu.1 Protein, pu1 Protein, sfpi1 Protein, si:by184l24.2 Protein, spi1 Protein, SPI1/PU.1 Protein, spi1l Protein, zgc:152991 Protein, Spi-1 proto-oncogene Protein, spleen focus forming virus (SFFV) proviral integration oncogene Protein, Spi-1 proto-oncogene b Protein, Spi-1 proto-oncogene a Protein, SPI1 Protein, Spi1 Protein, spi1b Protein, spi1a Protein

Background: SPI1 also known as transcription factor Pu.1, is a protein that in humans is encoded by the SPI1 gene. This protein have ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the Pu-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. It can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant SPI1, fused to His-tag at N-terminus, was expressed in E.coli.

Molecular Weight: 33.6kDa (294aa)

NCBI Accession: NP_001074016

UniProt: P17947

Pathways: Stem Cell Maintenance