AKR1B1 Protein (AA 1-316) (His tag)
-
- Target See all AKR1B1 Proteins
- AKR1B1 (Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-316
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This AKR1B1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMASHLEL NNGTKMPTLG LGTWKSPPGQ VTEAVKVAID LGYRHIDCAQ VYQNEKEVGV ALQEKLKEQV VKRQDLFIVS KLWCTFHDKS MVKGAFQKTL SDLQLDYLDL YLIHWPTGFK PGPDYFPLDA SGNVIPSDTD FVDTWTAMEQ LVDEGLVKTI GVSNFNPLQI ERILNKPGLK YKPAVNQIEC HPYLTQEKLI EYCHSKGIVV TAYSPLGSPD RPWAKPEDPS LLEDPRIKAI AAKYNKTTAQ VLIRFPIQRN LVVIPKSVTP VRIAENLKVF DFEVSSEDMA TLLSYNRNWR VCALMSCAKH KDYPFHAEV
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.
- Top Product
- Discover our top product AKR1B1 Protein
-
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Bioactivity Validated
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In Phosphate buffer saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- AKR1B1 (Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))
- Alternative Name
- Akr1b1 (AKR1B1 Products)
- Synonyms
- ADR Protein, ALDR1 Protein, ALR2 Protein, AR Protein, ALDRED Protein, ALR-P-I Protein, Akr1b3 Protein, Akr1b4 Protein, Aldr1 Protein, Alr Protein, RATALDRED Protein, akr1b7 Protein, zgc:86611 Protein, aldo-keto reductase family 1 member B Protein, aldo-keto reductase family 1, member B1 (aldose reductase) Protein, aldo-keto reductase family 1, member B1 (aldose reductase) S homeolog Protein, AKR1B1 Protein, Akr1b1 Protein, akr1b1.S Protein, akr1b1 Protein
- Background
- Akr1b1, also known as Aldose reductas, is an enzyme. It catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Recombinant mouse Akr1b1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 38.1kDa (339aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_033788
- UniProt
- P45376
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process
-